A PCR-based method for identification of lactobacilli at the genus level.

@article{Dubernet2002APM,
  title={A PCR-based method for identification of lactobacilli at the genus level.},
  author={S{\'e}gol{\`e}ne Dubernet and Nathalie Desmasures and Micheline Gu{\'e}guen},
  journal={FEMS microbiology letters},
  year={2002},
  volume={214 2},
  pages={271-5}
}
We developed a polymerase chain reaction (PCR)-based method for the identification of lactobacilli at the genus level. One specific primer, LbLMA1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16S and 23S rRNA genes in a number of Lactobacillus strains. Amplification with LbLMA1-rev and R16-1, a universal primer, generated a PCR product for 23 Lactobacillus species. Electrophoresis did not reveal any discrete bands when Escherichia coli… CONTINUE READING
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