A PCR-based epidemiological survey of Hepatozoon canis in dogs in Nigeria.

@article{Sasaki2008APE,
  title={A PCR-based epidemiological survey of Hepatozoon canis in dogs in Nigeria.},
  author={Mizuki Sasaki and Olutayo Temidayo Omobowale and Kaisaku Ohta and Morito Tozuka and Aya Matsuu and Haruyuki Hirata and Helen Oyebukola Nottidge and Hiromi Ikadai and Takashi Oyamada},
  journal={The Journal of veterinary medical science},
  year={2008},
  volume={70 7},
  pages={743-5}
}
The prevalence of Hepatozoon canis infections in dogs in Nigeria was surveyed using molecular methods. DNA was extracted from blood samples obtained from 400 dogs. A primer set that amplified the Babesia canis 18S rRNA gene, which has high similarity to the H. canis 18S rRNA gene, was used for the PCR. As a result, samples from 81 dogs (20.3%) produced 757 bp bands, which differed from the 698 bp band that corresponded to B. canis infection. The sequence of the PCR products of 10 samples were… CONTINUE READING
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