A PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropods.

@article{Rejili2016APD,
  title={A PCR-based diagnostic assay for detecting DNA of the olive fruit fly, Bactrocera oleae, in the gut of soil-living arthropods.},
  author={Mokhtar Rejili and T{\^a}nia Al{\'i}cia Ribeiro Fernandes and Ana Maria Dinis and Jos{\'e} A. Pereira and Paula Baptista and S{\'o}nia A. P. Santos and Teresa Lino-Neto},
  journal={Bulletin of entomological research},
  year={2016},
  volume={106 5},
  pages={
          695-9
        }
}
Bactrocera oleae (Rossi) (Diptera: Tephritidae) is considered the most devastating pest of the olive tree worldwide. In an effort to develop management and biological control strategies against this pest, new molecular tools are urgently needed. In this study, we present the design of B. oleae-specific primers based on mitochondrial DNA sequences of cytochrome oxidase subunit I (COI) gene. Two pairs of B. oleae-specific primers were successfully designed and named as SBo1-F/SBo1-R and SBo2-F… CONTINUE READING

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