A PCR-based assay for detection of emerging vaccinia-like viruses isolated in Brazil.

@article{Damaso2007APA,
  title={A PCR-based assay for detection of emerging vaccinia-like viruses isolated in Brazil.},
  author={Clarissa R Damaso and Sheila A Reis and Desyree Murta Jesus and Patr{\'i}cia S F Lima and Nissin Moussatch{\'e}},
  journal={Diagnostic microbiology and infectious disease},
  year={2007},
  volume={57 1},
  pages={39-46}
}
We report 2 strategies to identify Brazilian vaccinia virus (VACV) isolates related to Cantagalo virus (CTGV) based on the amplification of the hemagglutinin (HA) gene by the polymerase chain reaction (PCR). One PCR protocol was combined with restriction analysis using the endonuclease SnaB I, generating a unique digestion pattern for CTGV amplicons. The… CONTINUE READING