A PCR assay and PCR-restriction fragment length polymorphism combination identifying the 3 primary Mycoplasma species causing mastitis.

@article{Boonyayatra2012APA,
  title={A PCR assay and PCR-restriction fragment length polymorphism combination identifying the 3 primary Mycoplasma species causing mastitis.},
  author={Sukolrat Boonyayatra and Lawrence K. Fox and Thomas E. Besser and Ashish A. Sawant and John M. Gay and Ziv Raviv},
  journal={Journal of dairy science},
  year={2012},
  volume={95 1},
  pages={
          196-205
        }
}
The focus of the current research was to develop real-time PCR assays with improved sensitivity and the capacity to simultaneously speciate the 3 most common mycoplasma mastitis agents: Mycoplasma bovis, Mycoplasma californicum, and Mycoplasma bovigenitalium. Real-time PCR was chosen because it provides rapid results. Partial 16S rRNA gene sequencing was used as the gold standard for evaluating candidate real-time PCR assays. To ascertain the real-time PCR assay specificity, reference strains… CONTINUE READING

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