A One Pot, One Step, Precision Cloning Method with High Throughput Capability

@article{Engler2008AOP,
  title={A One Pot, One Step, Precision Cloning Method with High Throughput Capability},
  author={Carola Engler and Romy Kandzia and Sylvestre Marillonnet},
  journal={PLoS ONE},
  year={2008},
  volume={3},
  pages={2020 - 2028}
}
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed protein. We have devised a simple and rapid subcloning strategy to transfer any DNA fragment of interest from an entry clone into an expression vector, without this shortcoming. The strategy is based on the use of type IIs restriction enzymes, which… CONTINUE READING
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Modification of the Creator recombination system for proteomics applications–improved expression by addition of splice sites

  • K Colwill, CD Wells, E Kelly, M Goudreault, K Hersi
  • BMC Biotechnology
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