A One Pot, One Step, Precision Cloning Method with High Throughput Capability

  title={A One Pot, One Step, Precision Cloning Method with High Throughput Capability},
  author={Carola Engler and Romy Kandzia and Sylvestre Marillonnet},
  journal={PLoS ONE},
  pages={2020 - 2028}
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed protein. We have devised a simple and rapid subcloning strategy to transfer any DNA fragment of interest from an entry clone into an expression vector, without this shortcoming. The strategy is based on the use of type IIs restriction enzymes, which… CONTINUE READING
Highly Influential
This paper has highly influenced 23 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Recent Discussions
This paper has been referenced on Twitter 1 time over the past 90 days. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.
356 Citations
14 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 356 extracted citations


Publications referenced by this paper.
Showing 1-10 of 14 references

Modification of the Creator recombination system for proteomics applications–improved expression by addition of splice sites

  • K Colwill, CD Wells, E Kelly, M Goudreault, K Hersi
  • BMC Biotechnology
  • 2006

Similar Papers

Loading similar papers…