A Novel Technique to Detect EGFR Mutations in Lung Cancer

@inproceedings{Liu2016ANT,
  title={A Novel Technique to Detect EGFR Mutations in Lung Cancer},
  author={Yuanbin Liu and Ting Lei and Zhiyu Liu and Yanbin Kuang and Jianxin Lyu and Qi Wang},
  booktitle={International journal of molecular sciences},
  year={2016}
}
Epidermal growth factor receptor (EGFR) gene mutations occur in multiple human cancers; therefore, the detection of EGFR mutations could lead to early cancer diagnosis. This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as the AS-RPA-PNA-SYBR (ARPS) system. The… CONTINUE READING