Angiotensin-converting enzyme (ACE) (EC 4.15.1) metabolizes many biologically active peptides and plays a key role in blood pressure regulation and vascular remodeling. Elevated ACE levels are associated with different cardiovascular and respiratory diseases.
METHODS AND RESULTS
Two Belgian families with a 8-16-fold increase in blood ACE level… (More)
Figure 5. Analysis of cDNA from subjects harboring the IVS25+1G.A mutation versus controls. cDNA analysis of PCR products resulting from the amplification of cDNA from subjects harboring the IVS25+1G.A mutation versus wild type controls, visualised after electrophoresis on a 2% ethidium-bromide stained agarose gel. Ladder; Lanes 2–3: No Template Control (NTC); Lanes 4–5: two wild-type subjects for the IVS25+1G.A mutation; Lanes 6–9 : subjects heterozygous for the IVS25+1G.A mutation. doi:10.1371/journal.pone.0059537.g005