A Novel Rp-hplc Method for the Quantification of Nepafenac in Formulation, Plasma(in Vitro)


A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Nepafenac in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5μm in particle size) at ambient temperature. The mobile phase consisted of Methanol: ACN: THF 50:40:10 (V/V). The UV detection… (More)