A Novel Method for the Assessment of Cellular Composition and Beta‐Cell Viability in Human Islet Preparations

@article{Ichii2005ANM,
  title={A Novel Method for the Assessment of Cellular Composition and Beta‐Cell Viability in Human Islet Preparations},
  author={H. Ichii and L. Inverardi and A. Pileggi and R. Molano and O. Cabrera and A. Caicedo and S. Messinger and Y. Kuroda and P. Berggren and C. Ricordi},
  journal={American Journal of Transplantation},
  year={2005},
  volume={5}
}
Current methodologies to evaluate islet cell viability are largely based on tests that assess the exclusion of DNA‐binding dyes. While these tests identify cells that have lost selective membrane permeability, they do not allow us to recognize apoptotic cells, which do not yet stain with DNA‐binding dyes. Furthermore, current methods of analysis do not discriminate between cell subsets in the preparation and, in particular, they do not allow for selectively defining β‐cell viability. 
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Quantitative analysis of cell composition and purity of human pancreatic islet preparations
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