Corpus ID: 7366949

A Novel LC-ELSD Method for the Quantification of 2-Deoxy D-Glucose UsingHILIC Chromatography

  title={A Novel LC-ELSD Method for the Quantification of 2-Deoxy D-Glucose UsingHILIC Chromatography},
  author={Lakshmi Narayana. Ch and D. V. Subrahmanyam and D P.K.},
A simple and novel method for the direct determination of 2-deoxy D-glucose (2DG) is developed, using a hydrophilic interaction chromatography (HILIC) and evaporative light scattering detection (ELSD). Altech Altima HP HILIC column (250 x 4.6 mm, 5μ particles) was used along with the mobile phase consisted of water and acetonitrile in a linear inverse gradient elution. The proposed method is capable of separating 2DG from its precursor D-Glucal (DGL) and starting material D-Glucose (DGS) with… Expand
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