A Novel Kinase Inhibitor of FADD Phosphorylation Chemosensitizes through the Inhibition of NF-κB

@article{Schinske2011ANK,
  title={A Novel Kinase Inhibitor of FADD Phosphorylation Chemosensitizes through the Inhibition of NF-$\kappa$B},
  author={Katrina A. Schinske and S. Nyati and Amjad P. Khan and T. Williams and T. Johnson and B. Ross and R. P{\'e}rez Tom{\'a}s and A. Rehemtulla},
  journal={Molecular Cancer Therapeutics},
  year={2011},
  volume={10},
  pages={1807 - 1817}
}
Fas-associated protein with death domain (FADD) is a cytosolic adapter protein essential for mediating death receptor–induced apoptosis. It has also been implicated in a number of nonapoptotic activities including embryogenesis, cell-cycle progression, cell proliferation, and tumorigenesis. Our recent studies have shown that high levels of phosphorylated FADD (p-FADD) in tumor cells correlate with increased activation of the antiapoptotic transcription factor NF-κB and is a biomarker for… Expand
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A cell-based reporter for FADD kinase activity is developed, optimized for a high-throughput screen (HTS), that measures bioluminescence in response to modulation of FADD Kinase activity in live cells. Expand
Phosphorylation of FADD at serine 194 by CKIalpha regulates its nonapoptotic activities.
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It is demonstrated that casein kinase Ialpha (CKIalpha) phosphorylates FADD at Ser194 both in vitro and in vivo, suggesting that phosphorylation of FADD by CKI is a crucial event during mitosis. Expand
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It is shown that the nuclear localization of FADD and elevated expression of the phosphorylated form of FAD correlated most closely with an increase in NF-κB activity and poor clinical outcome, suggesting that levels of p-FADD may be used as a prognostic biomarker for predicting survival of lung cancer patients. Expand
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