A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

@inproceedings{Sailon2009ANF,
  title={A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic  Cell Culture},
  author={Alexander M. Sailon and Alexander Christopher Allori and Edward H. Davidson and Derek Daniel Reformat and Robert J. Allen and Stephen M. Warren},
  booktitle={Journal of biomedicine & biotechnology},
  year={2009}
}
BACKGROUND Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. METHODS Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution… CONTINUE READING
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