A Novel Binding Assay For Metabotropic Glutamate Receptors Using [3H] ʟ-Quisqualic Acid And Recombinant Receptors

@article{Ohashi2002ANB,
  title={A Novel Binding Assay For Metabotropic Glutamate Receptors Using [3H] ʟ-Quisqualic Acid And Recombinant Receptors},
  author={Hiroshi Ohashi and Takaharu Maruyama and Hidemi Higashi-Matsumoto and Takashi Nomoto and Yutaka Takeuchi},
  journal={Zeitschrift f{\"u}r Naturforschung C},
  year={2002},
  volume={57},
  pages={348 - 355}
}
We established a methodology to analyze radioligand binding to the recombinant type 1a metabotropic glutamate receptor (mGluR1a). A full-length ᴄDNA encoding mGluR1a, which was isolated from a λ gt 11 ᴄDNA library of human cerebellar origin, was expressed in a baculovirus/Sf9 insect cell system. Membrane fractions with recombinant receptor expression were analyzed for the binding of [3H]L-quisqualic acid (ʟ-QA), which is known to be a potent agonist of mGluR1a. Efficient binding of the… Expand
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