A Novel, Evolutionarily Conserved Protein Phosphatase Complex Involved in Cisplatin Sensitivity*S

  title={A Novel, Evolutionarily Conserved Protein Phosphatase Complex Involved in Cisplatin Sensitivity*S},
  author={Anne-Claude Gingras and Michael Caballero and Marcel Zarske and Amy Sanchez and Tony R. Hazbun and Stanley Fields and Nahum Sonenberg and Ernst Hafen and Brian Raught and Ruedi Aebersold},
  journal={Molecular \& Cellular Proteomics},
  pages={1725 - 1740}
Using a combination of tandem affinity purification tagging and mass spectrometry, we characterized a novel, evolutionarily conserved protein phosphatase 4 (PP4)-containing complex (PP4cs, protein phosphatase 4, cisplatin-sensitive complex) that plays a critical role in the eukaryotic DNA damage response. PP4cs is comprised of the catalytic subunit PP4C; a known regulatory subunit, PP4R2; and a novel protein that we termed PP4R3. The Saccharomyces cerevisiae PP4R3 ortholog Psy2 was identified… 

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