A Note on the use of Steady–State Fluorescence Quenching to Quantify Nanoparticle–Protein Interactions

Abstract

Steady–state fluorescence quenching is a commonly used technique to investigate the interactions between proteins and nanoparticles, providing quantitative information on binding affinity, stoichiometry and cooperativity. However, a failure to account for the limitations and pitfalls of the methodology can lead to significant errors in data analysis and… (More)
DOI: 10.1007/s10895-015-1665-3

5 Figures and Tables

Cite this paper

@article{Sousa2015ANO, title={A Note on the use of Steady–State Fluorescence Quenching to Quantify Nanoparticle–Protein Interactions}, author={Alioscka A. Sousa}, journal={Journal of Fluorescence}, year={2015}, volume={25}, pages={1567-1575} }