A Newly Uncovered Group of Distantly Related Lysine Methyltransferases Preferentially Interact with Molecular Chaperones to Regulate Their Activity
@article{Cloutier2013ANU, title={A Newly Uncovered Group of Distantly Related Lysine Methyltransferases Preferentially Interact with Molecular Chaperones to Regulate Their Activity}, author={Philippe Cloutier and Mathieu Lavall{\'e}e-Adam and Denis Faubert and Mathieu Blanchette and Benoit Coulombe}, journal={PLoS Genetics}, year={2013}, volume={9} }
Methylation is a post-translational modification that can affect numerous features of proteins, notably cellular localization, turnover, activity, and molecular interactions. Recent genome-wide analyses have considerably extended the list of human genes encoding putative methyltransferases. Studies on protein methyltransferases have revealed that the regulatory function of methylation is not limited to epigenetics, with many non-histone substrates now being discovered. We present here our…
136 Citations
Identification and Characterization of a Novel Human Methyltransferase Modulating Hsp70 Protein Function through Lysine Methylation*
- BiologyThe Journal of Biological Chemistry
- 2013
It is shown that trimethylation of HSPA8 (Hsc70) has functional consequences, as it alters the affinity of the chaperone for both the monomeric and fibrillar forms of the Parkinson disease-associated protein α-synuclein.
Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation
- BiologyNucleic acids research
- 2017
It is demonstrated that an uncharacterized human 7BS MTase currently annotated as part of the endothelin converting enzyme 2, but which should be considered a separate enzyme efficiently methylates K36 in eukaryotic translation elongation factor 1 alpha (eEF1A) in vitro and in vivo.
Human METTL18 is a histidine-specific methyltransferase that targets RPL3 and affects ribosome biogenesis and function
- BiologyNucleic acids research
- 2021
METTL18 is established as the second human histidine-specific protein MTase, and its functional relevance is demonstrated, indicating that METTL18-mediated methylation of RPL3 is important for optimal ribosome biogenesis and function.
A System for Enzymatic Lysine Methylation in a Desired Sequence Context
- BiologyACS omega
- 2017
A versatile system for introducing lysine methylation into a desired peptide sequence is described, and the approach should be readily expandable for generating combinatorial libraries of methylated sequences.
Identification and Characterization of a Novel Evolutionarily Conserved Lysine-specific Methyltransferase Targeting Eukaryotic Translation Elongation Factor 2 (eEF2)*
- BiologyThe Journal of Biological Chemistry
- 2014
The present study establishes the function of the previously uncharacterized MTases FAM86A and Yjr129c, demonstrating that these enzymes introduce a functionally important lysine methylation in eEF2.
Elongation factor methyltransferase 3--a novel eukaryotic lysine methyltransferase.
- Biology, ChemistryBiochemical and biophysical research communications
- 2014
Protein lysine methylation by seven-β-strand methyltransferases.
- Biology, ChemistryThe Biochemical journal
- 2016
A number of novel 7BS KMTs have now been discovered, and, in particular, several recently characterized human and yeast members of MTase family 16 (MTF16) have been found to methylate lysines in non-histone proteins.
Regulation of eukaryotic elongation factor 1 alpha (eEF1A) by dynamic lysine methylation
- BiologyRNA biology
- 2018
It is shown that lysine methylation of eEF1A can be dynamic and inducible, and modulates mRNA translation in a codon-specific fashion.
Selenium-Based S-Adenosylmethionine Analog Reveals the Mammalian Seven-Beta-Strand Methyltransferase METTL10 to Be an EF1A1 Lysine Methyltransferase
- BiologyPloS one
- 2014
The utility of the synthetic cofactor ProSeAM as a chemical probe for the identification of non-histone substrates of KMTs of lysine methylation is emphasized.
Methylation of the DNA/RNA-binding protein Kin17 by METTL22 affects its association with chromatin.
- BiologyJournal of proteomics
- 2014
References
SHOWING 1-10 OF 92 REFERENCES
Methylation of H3-Lysine 79 Is Mediated by a New Family of HMTases without a SET Domain
- Biology, ChemistryCurrent Biology
- 2002
NRMT is an α-N-methyltransferase that methylates RCC1 and Retinoblastoma Protein
- Biology, ChemistryNature
- 2010
The post-translational methylation of α-amino groups was first discovered over 30 years ago on the bacterial ribosomal proteins L16 and L33 (refs 1, 2), but almost nothing is known about the function…
A Novel 3-Methylhistidine Modification of Yeast Ribosomal Protein Rpl3 Is Dependent upon the YIL110W Methyltransferase*
- BiologyThe Journal of Biological Chemistry
- 2010
It is shown that Rpl3, a protein of the large ribosomal subunit from baker's yeast, is stoichiometrically monomethylated at position 243, producing a 3-methylhistidine residue, the first report of a methylated histidine residue in yeast cells, and the first example of a gene required for protein histidine methylation in nature.
Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln
- Biology, ChemistryCurrent Biology
- 2001
Identification and validation of eukaryotic aspartate and glutamate methylation in proteins.
- Biology, ChemistryJournal of proteome research
- 2008
This is the first report conclusively identifying in vivo D/E-methylation substrates and their modification sites in eukaryotic cells, demonstrating that D/ E-methylations are abundant protein modifications.
Determination of enriched histone modifications in non-genic portions of the human genome
- BiologyBMC Genomics
- 2008
This work has extended the analysis of histone modifications to gene deserts, pericentromeres and subtelomeres and found that each of these non-genic regions has a particular profile of hist one modifications that distinguish it from the other non-coding regions.
Repression of p53 activity by Smyd2-mediated methylation
- Biology, ChemistryNature
- 2006
A lysine methyltransferase, Smyd2, is reported that methylates a previously unidentified site, Lys 370, in p53, which is repressing to p53-mediated transcriptional regulation and providing regulatory cross-talk between post-translational modifications.
Uncovering the Human Methyltransferasome*
- Biology, Computer ScienceMolecular & Cellular Proteomics
- 2010
Primary sequences, predicted secondary structures, and solved crystal structures of known methyltransferases were analyzed by hidden Markov models, Fisher-based statistical matrices, and fold recognition prediction-based threading algorithms to create a model, or profile, of each methyltransferase superfamily.
Calmodulin methyltransferase is an evolutionarily conserved enzyme that trimethylates Lys-115 in calmodulin.
- BiologyNature communications
- 2010
A class I, non-SET domain protein methyltransferase, calmodulin-lysine N-methyltransferase (EC 2.1.60) is identified, implicating a broad role for lysine methylation in calcium-dependent signalling.
p97/valosin‐containing protein (VCP) is highly modulated by phosphorylation and acetylation
- Biology, ChemistryGenes to cells : devoted to molecular & cellular mechanisms
- 2009
It is proposed that D2α domain acts as a VCP ATPase Regulatory domain or “VAR domain” and modifications including those in this VAR domain may endorse adaptive and multiple functions to VCP in different cell conditions such as in the cell cycle and with abnormal protein accumulation.