• Corpus ID: 37333453

A New and Sensitive LC-MS/MS Method for the Determination of Clopidogrel in Human Plasma

@inproceedings{Bibire2014ANA,
  title={A New and Sensitive LC-MS/MS Method for the Determination of Clopidogrel in Human Plasma},
  author={Nela Bibire and Mădălina Vieriu and Gladiola Ț{\^a}ntaru and Mihai Apostu and Luminița Agoroaei and Alina Diana Panainte and Alexandru Znagovan and Aurelian Vlase and Grigore T. Popa},
  year={2014}
}

• N. Bibire, Mădălina Vieriu, +6 authors G. Popa
• Published 2014
• Biology, Chemistry

→ → → → 212.1, for clopidogrel and m/z 327 → 217, for clopidogrel-d3, in multiple reaction monitoring mode (MRM). The plasma samples were buffered (pH 2.0), extracted using a mixture hexan-ethyl acetate and then 10μL of the sample extract was injected into the LC-MS/MS system. Clopidogrel and the internal standard were separated on a Zorbax SB-C8 column using a mobile phase consisting of 50:50 (v/v) mixture of acetonitrile and 0.1% formic acid aqueous solution, with a flow rate of 1 mL/min. The proposed method has been validated for a linear range of 10-10000 mg/mL and the value of the regression coefficient was ≥0.99. The precision (CV%) and accuracy (%) were lower than 6.1 % for intra- and inter-days assays. The overall recoveries for clopidogrel and clopidogrel-d3 were 81 and 84%, respectively. The overall time of the analysis was 7 min. The method is sensitive, specific and reproducible and can be used for the therapeutic drug monitoring of clopidogrel.