A New Workflow for Whole‐Genome Sequencing of Single Human Cells

  title={A New Workflow for Whole‐Genome Sequencing of Single Human Cells},
  author={V. Binder and Christoph Bartenhagen and V. Okpanyi and M. Gombert and Birte Moehlendick and B. Behrens and H. Klein and H. Rieder and Pina Fanny Ida Krell and M. Dugas and N. H. Stoecklein and A. Borkhardt},
  journal={Human Mutation},
  • V. Binder, Christoph Bartenhagen, +9 authors A. Borkhardt
  • Published 2014
  • Biology, Medicine
  • Human Mutation
  • Unbiased amplification of the whole‐genome amplification (WGA) of single cells is crucial to study cancer evolution and genetic heterogeneity, but is challenging due to the high complexity of the human genome. Here, we present a new workflow combining an efficient adapter‐linker PCR‐based WGA method with second‐generation sequencing. This approach allows comparison of single cells at base pair resolution. Amplification recovered up to 74% of the human genome. Copy‐number variants and loss of… CONTINUE READING
    19 Citations
    The Performance of Whole Genome Amplification Methods and Next-Generation Sequencing for Pre-Implantation Genetic Diagnosis of Chromosomal Abnormalities.
    • N. Li, L. Wang, +7 authors Y. Yao
    • Biology, Medicine
    • Journal of genetics and genomics = Yi chuan xue bao
    • 2015
    • 25
    Deterministic Whole-Genome Amplification of Single Cells.
    • 3
    Next-generation molecular diagnosis: single-cell sequencing from bench to bedside
    • 15
    Simultaneous detection of BRCA mutations and large genomic rearrangements in germline DNA and FFPE tumor samples
    • 16
    • PDF
    Principles guiding embryo selection following genome-wide haplotyping of preimplantation embryos
    • 17
    • PDF
    Using fetal cells for prenatal diagnosis: History and recent progress
    • A. Beaudet
    • Biology, Medicine
    • American journal of medical genetics. Part C, Seminars in medical genetics
    • 2016
    • 28


    Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell
    • 580
    • Highly Influential
    • PDF
    Single-cell paired-end genome sequencing reveals structural variation per cell cycle
    • 127
    • Highly Influential
    • PDF
    Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells.
    • 435
    • Highly Influential
    • PDF
    Whole Genome Amplification and De novo Assembly of Single Bacterial Cells
    • 230
    • PDF
    Tumour evolution inferred by single-cell sequencing
    • 1,950
    • PDF
    A map of human genome variation from population-scale sequencing
    • 6,526
    • Highly Influential
    • PDF
    Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm
    • 468
    • Highly Influential
    • PDF
    Use of high throughput sequencing to observe genome dynamics at a single cell level
    • 38
    • PDF
    Single-cell sequencing
    • Tal Nawy
    • Medicine, Biology
    • Nature Methods
    • 2013
    • 101