A New Workflow for Whole‐Genome Sequencing of Single Human Cells

@article{Binder2014ANW,
  title={A New Workflow for Whole‐Genome Sequencing of Single Human Cells},
  author={V. Binder and Christoph Bartenhagen and V. Okpanyi and M. Gombert and Birte Moehlendick and B. Behrens and H. Klein and H. Rieder and Pina Fanny Ida Krell and M. Dugas and N. H. Stoecklein and A. Borkhardt},
  journal={Human Mutation},
  year={2014},
  volume={35}
}
  • V. Binder, Christoph Bartenhagen, +9 authors A. Borkhardt
  • Published 2014
  • Biology, Medicine
  • Human Mutation
  • Unbiased amplification of the whole‐genome amplification (WGA) of single cells is crucial to study cancer evolution and genetic heterogeneity, but is challenging due to the high complexity of the human genome. Here, we present a new workflow combining an efficient adapter‐linker PCR‐based WGA method with second‐generation sequencing. This approach allows comparison of single cells at base pair resolution. Amplification recovered up to 74% of the human genome. Copy‐number variants and loss of… CONTINUE READING
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