A New Sendai Virus Vector Deficient in the Matrix Gene Does Not Form Virus Particles and Shows Extensive Cell-to-Cell Spreading

@article{Inoue2003ANS,
  title={A New Sendai Virus Vector Deficient in the Matrix Gene Does Not Form Virus Particles and Shows Extensive Cell-to-Cell Spreading},
  author={Makoto Inoue and Yumiko Tokusumi and Hiroshi Ban and Takumi Kanaya and Masayuki Shirakura and Tsuyoshi Tokusumi and Takahiro Hirata and Yoshiyuki Nagai and Akihiro Iida and Mamoru Hasegawa},
  journal={Journal of Virology},
  year={2003},
  volume={77},
  pages={6419 - 6429}
}
ABSTRACT A new recombinant Sendai virus vector (SeV/ΔM), in which the gene encoding matrix (M) protein was deleted, was recovered from cDNA and propagated in a packaging cell line expressing M protein by using a Cre/loxP induction system. The titer of SeV/ΔM carrying the enhanced green fluorescent protein gene in place of the M gene was 7 × 107 cell infectious units/ml or more. The new vector showed high levels of infectivity and gene expression, similar to those of wild-type SeV vector, in… 
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