A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus but Does Not Replicate in Vertebrate Cells

@article{Harrison2016ANO,
  title={A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus but Does Not Replicate in Vertebrate Cells},
  author={J. Harrison and D. Warrilow and Breeanna J. McLean and D. Watterson and Caitlin A O'Brien and Agathe M G Colmant and C. Johansen and R. Barnard and S. Hall-Mendelin and S. Davis and R. Hall and J. Hobson-Peters},
  journal={Viruses},
  year={2016},
  volume={8}
}
The discovery and characterisation of new mosquito-borne viruses provides valuable information on the biodiversity of vector-borne viruses and important insights into their evolution. In this study, a broad-spectrum virus screening system, based on the detection of long double-stranded RNA in inoculated cell cultures, was used to investigate the presence of novel viruses in mosquito populations of northern Australia. We detected and isolated a new virus (tentatively named Parry’s Lagoon virus… Expand
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References

SHOWING 1-10 OF 52 REFERENCES
A New Insect-Specific Flavivirus from Northern Australia Suppresses Replication of West Nile Virus and Murray Valley Encephalitis Virus in Co-infected Mosquito Cells
TLDR
It is demonstrated that prior infection of cultured mosquito cells with PCV suppressed subsequent replication of the medically significant West Nile and Murray Valley encephalitis viruses by 10–43 fold, suggesting that superinfection exclusion can occur between ISFs and vertebrate-infecting flaviviruses despite their high level of genetic diversity. Expand
A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia.
TLDR
Investigation of the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR and revealed PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Expand
Expansion of family Reoviridae to include nine-segmented dsRNA viruses: isolation and characterization of a new virus designated Aedes pseudoscutellaris reovirus assigned to a proposed genus (Dinovernavirus).
TLDR
It is suggested that APRV should be classified within a new genus that is designated Dinovernavirus (sigla from D: Double-stranded, i: insect, nove: nine from the latin "novem", rna: RNA, virus) in family Reoviridae. Expand
Enhanced arbovirus surveillance with deep sequencing: Identification of novel rhabdoviruses and bunyaviruses in Australian mosquitoes.
TLDR
A metagenomics approach was used to identify virus sequences in Australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures, revealing strains of Liao Ning virus, livestock-infecting Stretch Lagoon virus, two novel dimarhabdoviruses, named Beaumont and North Creek viruses, and two novel orthobunyaviruses. Expand
A Newly Isolated Reovirus Has the Simplest Genomic and Structural Organization of Any Reovirus
TLDR
Fako virus, tentatively designated Fako virus (FAKV), represents the first 9-segment, double-stranded RNA (dsRNA) virus to be isolated in nature and appears to have a broad mosquito host range, and its detection in male specimens suggests mosquito-to-mosquito transmission in nature. Expand
Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia.
TLDR
Phylogenetic analysis showed that SLOV should be considered a new species within the genus Orbivirus, and a real-time RT-PCR test was designed to study the epidemiology of SLOV in the field. Expand
Characterization of Rabensburg Virus, a Flavivirus Closely Related to West Nile Virus of the Japanese Encephalitis Antigenic Group
TLDR
Evaluating vector competence and capacity for vertical transmission in Cx. Expand
Yunnan orbivirus, a new orbivirus species isolated from Culex tritaeniorhynchus mosquitoes in China.
TLDR
The replication of YUOV in mosquito cell lines was restricted to Aedes albopictus cells and the virus failed to replicate in mammalian cell lines, however, intraperitoneal injection of virus into naïve mice resulted in productive, non-lethal virus replication and viraemia. Expand
Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.
TLDR
The PNG isolates belonged to genotype II, and shared > 99.2% identity with isolates from humans and mosquitoes from the Torres Strait, suggesting that PNG is the source of incursions of JE virus into Australia. Expand
Complete sequence characterization of the genome of the St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis.
TLDR
Blot hybridizations and sequence comparisons of the conserved protein encoded by genome segment 2 (the T2 subcore shell protein) with previously identified orbiviruses confirm that SCRV is a distinct orbivirus species, unrelated to another tick-borne species, Great Island virus. Expand
...
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4
5
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