A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm
@article{Kojima2007ANM, title={A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm}, author={K. Kojima and Y. Kuwana and H. Sezutsu and I. Kobayashi and K. Uchino and T. Tamura and Y. Tamada}, journal={Bioscience, Biotechnology, and Biochemistry}, year={2007}, volume={71}, pages={2943 - 2951} }
We constructed a new plasmid vector for the production of a modified silk fibroin heavy chain protein (H-chain) in the transgenic silkworm. The plasmid (pHC-null) contained the promoter and the 3′ region of a gene encoding the H-chain and the coding regions for the N-terminal domain and the C-terminal domain of the H-chain. For the model protein, we cloned a foreign gene that encoded EGFP between the N-terminal domain and the C-terminal domain in pHC-null and generated transgenic silkworms that… CONTINUE READING
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