A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm

@article{Kojima2007ANM,
  title={A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm},
  author={Katsura Kojima and Yoshihiko Kuwana and Hideki Sezutsu and Isao Kobayashi and Keiro Uchino and Toshiki Tamura and Yasushi Tamada},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2007},
  volume={71},
  pages={2943 - 2951}
}
We constructed a new plasmid vector for the production of a modified silk fibroin heavy chain protein (H-chain) in the transgenic silkworm. The plasmid (pHC-null) contained the promoter and the 3′ region of a gene encoding the H-chain and the coding regions for the N-terminal domain and the C-terminal domain of the H-chain. For the model protein, we cloned a foreign gene that encoded EGFP between the N-terminal domain and the C-terminal domain in pHC-null and generated transgenic silkworms that… 

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...

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