A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins

@inproceedings{ManKupisinska2016ANL,
  title={A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins},
  author={Aleksandra Man-Kupisinska and Mateusz Michalski and Anna Maciejewska and Anna Stanisława Świerzko and Maciej Cedzyński and Czeslaw Lugowski and Jolanta Lukasiewicz},
  booktitle={PloS one},
  year={2016}
}
Despite recombinant protein technology development, proteins isolated from natural sources remain important for structure and activity determination. Ficolins represent a class of proteins that are difficult to isolate. To date, three methods for purifying ficolin-3 from plasma/serum have been proposed, defined by most critical step: (i) hydroxyapatite… CONTINUE READING