A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin.
@article{Neeliolu2017ANK, title={A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin.}, author={S. Neşelioğlu and M. Ergin and O. Erel}, journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry}, year={2017}, volume={33 12}, pages={ 1339-1344 } }
A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001… CONTINUE READING
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