A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology.

  title={A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology.},
  author={Abdelrahman Saleh Zaky and Darren Greetham and Edward J Louis and G. A. Tucker and Chenyu Du},
  journal={Journal of microbiology and biotechnology},
  volume={26 11},
  • A. Zaky, D. Greetham, C. Du
  • Published 28 November 2016
  • Biology, Engineering
  • Journal of microbiology and biotechnology
Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. [] Key Method The new method includes a three-cycle enrichment step followed by an isolation step and a confirmation step. Using this method, 116 marine yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA.

Figures and Tables from this paper

Occurrence and Distribution of Strains of Saccharomyces cerevisiae in China Seas
It was found that most of the strains of S. cerevisiae in marine environments occurred in guts, the surface of marine fish and mangrove trees, in contrast, they were not found in seawater and sediments, and may be related to the harsh marine environments from which they were isolated.
Utilisation of novel marine yeast and seawater-based media for the production of bioethanol
Bioethanol can be considered as one of the best replacements for petrol because of its positive impact on environment and many other advantages. Currently, bioethanol accounts for around 2% of the
The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain
Investigation of the use of seawater-based media and a novel marine yeast strain ‘Saccharomyces cerevisiae AZ65’ to reduce the water footprint of bioethanol and revealed that marine yeast is a potential candidate for use in theBioethanol industry especially when using seawater or high salt based fermentation media.
Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626
This work put forward C. membranifaciens IST 626 as a riboflavin overproducer and provides valuable molecular data for future development of superior producing strains capable of using the wide range of carbon sources, which is a characteristic trait of the species.
Aquatic yeasts: diversity, characteristics and potential health implications.
There has been a rising interest in the levels, diversity and potential impacts of yeasts in aquatic environments, and there is a need to review the literature that also focuses on aquatic environments.
Biodiversity and Enzyme Activity of Marine Fungi with 28 New Records from the Tropical Coastal Ecosystems in Vietnam
Abstract The coastal marine ecosystems of Vietnam are one of the global biodiversity hotspots, but the biodiversity of marine fungi is not well known. To fill this major gap of knowledge, we assessed
Transcriptional profile of a bioethanol production contaminant Candida tropicalis
The expression pattern suggested that the functional and transcriptional behavior of the contaminating yeast during fermentation for bioethanol production is similar to that of the standard yeast Saccharomyces cerevisiae, and confirmed that C. tropicalis is an important contaminant of the alcoholic fermentation process.
Production, purification, characterization, and biological properties of Rhodosporidium paludigenum polysaccharide
Marine red yeast polysaccharide obtained from Rhodosporidium paludigenum improved the lambs’ body weight, antioxidant enzyme activity, and the number of probiotics, but it reduced the feed/gain (F/G) ratio and theNumber of pathogenic bacteria in 60-days-old lambs.
Saccharification of lignocellulosic biomass using seawater and halotolerant cellulase with potential application in second-generation bioethanol production
This study involves marine bacterial strains Bacillus oceanisediminis, Brevibacterium halotolerans, and Psychrobacter celer capable of producing halotoleranceant cellulases, isolated from Gopalpur, Odisha, and utilized for saccharification of pretreated rice straw and the production of reducing sugars.


The isolation of novel marine yeasts; a new procedure
Development of a simple isolation method for yeast Saccharomyces cerevisiae with high fermentative activities from coastal waters
The yeast species S. cerevisiae was found to be simply isolated from coastal waters by the method and ethanol productivities of 10 strains randomly selected from the 29 ones were measured, and there was an almost linear relationship between ethanol and gas productivities in the 10 strains.
Collection and identification of marine yeasts
Development of a phenotypic assay for characterisation of ethanologenic yeast strain sensitivity to inhibitors released from lignocellulosic feedstocks
The results demonstrate that the phenotypic microarray assay developed in the current study is a valuable tool that can be used to identify yeast strains with desirable resistance to inhibitory compounds found in lignocellulosic hydrolysates.
Bioethanol from Macroalgal Biomass: Utilization of Marine Yeast for Production of the Same
The ability of this marine yeast to function at high salinity can be commercialized for its use to convert seaweed polysaccharide based hydrolysate, rich in salt, to ethanol without desalting process, ultimately making the process more efficient and economically viable.
Marine yeasts—a review
The distribution of species, as well as their numbers and metabolic characteristics were found to be governed by existing environmental conditions, and yeasts are more abundant in silty muds than in sandy sediments.
Screening of Non- Saccharomyces cerevisiae Strains for Tolerance to Formic Acid in Bioethanol Fermentation
This study investigated the potential for screening formic acid tolerance in non-Saccharomyces cerevisiae yeast strains, which could be used for the development of advanced generation bioethanol processes, and found S. arboricolus strain 2.3319 displayed a higher formic Acid tolerance when compared to other strains in the study.
Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide
Strain BM39, probably belonging to a new species, due to its remarkable EPS production, comparable to those of known industrial bacterial producers, could be suggested as a new microorganism for industrial applications.