A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei

@inproceedings{Sunter2012ANG,
  title={A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei},
  author={Jack Sunter and Bill Wickstead and Keith Gull and Mark Carrington},
  booktitle={PloS one},
  year={2012}
}
Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator to prevent transcription in the absence of tetracycline. The same inducible system is used to produce double-stranded RNA for RNAi knockdown of target genes. This study describes a new plasmid pSPR2.1 that drives consistent high-level expression of tetracycline repressor… CONTINUE READING
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