Corpus ID: 29925867

A Nanosensor System Based On Disuccinimydyl–CYP2E1 for Amperometric Detection of the Anti-Tuberculosis Drug, Pyrazinamide

  title={A Nanosensor System Based On Disuccinimydyl–CYP2E1 for Amperometric Detection of the Anti-Tuberculosis Drug, Pyrazinamide},
  author={Rachel Fanelwa Ajayi and Unathi Sidwaba and Usisipho Feleni and Samantha Fiona Douman and Ezo Nxusani and Lindsay Wilson and Candice Rassie and Oluwakemi Tovide and Priscilla Gloria Lorraine Baker and Sibulelo L. Vilakazi and Robert Tshikhudo and Emmanuel I. Iwuoha},
  journal={World Academy of Science, Engineering and Technology, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering},
  • R. F. Ajayi, Unathi Sidwaba, +9 authors E. Iwuoha
  • Published 3 January 2014
  • Chemistry, Medicine
  • World Academy of Science, Engineering and Technology, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering
Pyrazinamide (PZA) is among the first-line pro-drugs in the tuberculosis (TB) combination chemotherapy used to treat Mycobacterium tuberculosis. Numerous reports have suggested that hepatotoxicity due to pyrazinamide in patients is due to inappropriate dosing. It is, therefore necessary to develop sensitive and reliable techniques for determining the PZA metabolic profile of diagnosed patients promptly and at point-of-care. This study reports the determination of PZA based on nanobiosensor… Expand
An Amperometric Cytochrome P450-2D6 Biosensor System for the Detection of the Selective Serotonin Reuptake Inhibitors (SSRIs) Paroxetine and Fluvoxamine
Paroxetine is the second most prescribed selective serotonin reuptake inhibitor (SSRI) antidepressant drug, characterized by extensive inter-individual variation in steady state plasma concentrationsExpand


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Ethambutol (2S, 2′S)-2, 2′-(ethane-1, 2-diyldiimino) dibutan-1-ol) is a first-line antitubercular drug effective against actively growing Mycobacterium tuberculosis. Resistance of the mycobacteriumExpand
The curious characteristics of pyrazinamide: a review.
  • Y. Zhang, D. Mitchison
  • Medicine
  • The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
  • 2003
Evidence from clinical trials is described that it is an effective sterilising drug that acts synergistically with rifampicin and the highly diverse mutations in the PZase gene (pncA) that lead to loss of Pzase activity cause PZA resistance. Expand
Determination of pyrazinamide and its main metabolites in rat urine by high-performance liquid chromatography.
A new high-performance liquid chromatograhic procedure for simultaneous determination of pyrazinamide (PZA) and its three metabolites 5-hydroxypyrazinamia, pyrazinoic acid, and 5-OH-PA, in rat urine was developed and will constitute a useful tool for studies on the influence of drug interactions in tuberculosis treatment. Expand
Pharmacokinetics-Pharmacodynamics of Pyrazinamide in a Novel In Vitro Model of Tuberculosis for Sterilizing Effect: a Paradigm for Faster Assessment of New Antituberculosis Drugs
An in vitro model for the sterilizing effect is designed in which Mycobacterium tuberculosis replicating slowly at pH 5.8 was exposed to pyrazinamide by use of the concentration-time profiles encountered in patients, and can be used for the faster identification of the clinical doses that are needed to achieve a sterilizingEffect. Expand
Determination of pyrazinamide MICs for Mycobacterium tuberculosis at different pHs by the radiometric method
The MICs of pyrazinamide (PZA) were determined for Mycobacterium tuberculosis cultivated under different pH conditions in 7H12 liquid medium by the radiometric method, and a predictable eightfold difference was observed between the MICs determined at pH 5.5 and those determined at GPA 5.95. Expand
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The cardinal features of the main enzymes of the MEP pathway in M. tuberculosis are described and how these can be manipulated in high throughput screening campaigns in the search for new anti-infectives against TB. Expand
Pyrazinamide Inhibits Trans-Translation in Mycobacterium tuberculosis
It is confirmed that POA bound to RpsA (but not a clinically identified ΔAla mutant) and subsequently inhibited trans-translation rather than canonical translation, and its inhibition may explain the ability of PZA to eradicate persisting organisms. Expand
Role of acid pH and deficient efflux of pyrazinoic acid in unique susceptibility of Mycobacterium tuberculosis to pyrazinamide.
In M. tuberculosis, acidic pH enhanced the intracellular accumulation of pyrazinoic acid (POA), the active derivative of PZA, after conversion of Pza by pyrazinamidase, and the natural PZA resistance of M. smegmatis is due to a highly active efflux pump. Expand
Electrochemical behavior of bisphenol A at glassy carbon electrode modified with gold nanoparticles, silk fibroin, and PAMAM dendrimers
A glassy carbon electrode was modified with a composite made from gold nanoparticles and silk fibroin whose surface was further modified with amino-terminated G4 poly(amidoamine) dendrimer. ThisExpand
Pyrazinoic acid efflux rate in Mycobacterium tuberculosis is a better proxy of pyrazinamide resistance.
This finding suggests that mechanisms other than pncA mutations (reduction of pyrazinamidase activity) are also implicated in pyraz inamide resistance, and that pyrazinoic acid efflux rate acts as a better proxy forpyrazinamide resistance than the presence of pNCA mutations. Expand