A Multiplexed Protein Kinase Assay

@article{Shults2007AMP,
  title={A Multiplexed Protein Kinase Assay},
  author={Melissa D Shults and I. Kozlov and Nicholas A Nelson and B. Kermani and P. Melnyk and V. Shevchenko and A. Srinivasan and Joseph Musmacker and J. Hachmann and D. Barker and M. Lebl and Chanfeng Zhao},
  journal={ChemBioChem},
  year={2007},
  volume={8}
}
We report a novel protein kinase assay designed for high‐throughput detection of one or many kinases in a complex mixture. A solution‐phase phosphorylation reaction is performed on 900 different peptide substrates, each covalently linked to an oligonucleotide tag. After incubation, phosphoserine, phosphothreonine, and phosphotyrosine are chemically labeled, and the substrates are hybridized to a microarray with oligonucleotides complementary to the tags to read out the phosphorylation state of… Expand
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