A Multidisciplinary Approach to the Study of T Cell Migration

@article{Long2004AMA,
  title={A Multidisciplinary Approach to the Study of T Cell Migration},
  author={Aideen Long and Siobhan Mitchell and Dmitry Kashanin and Vivienne Williams and Adriele Prina Mello and Igor V. Shvets and Dermot Kelleher and Yuri Volkov},
  journal={Annals of the New York Academy of Sciences},
  year={2004},
  volume={1028}
}
Abstract: Active T cell locomotion depends on efficient repeated cycles of integrin receptor/ligand interactions mediating cell adhesion and detachment, intracellular signaling cascades orchestrating posttranslation modifications of interacting proteins, dynamic reassembly of participating cytoskeletal elements, and structural support of associated scaffolding molecules. Using an integrated approach based on novel cutting edge technologies of live cell imaging, cell transfection, proteomics… Expand
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References

SHOWING 1-5 OF 5 REFERENCES
Crucial importance of PKC-beta(I) in LFA-1-mediated locomotion of activated T cells.
TLDR
It is shown that PKC-beta(I) is a component of the tubulin-enriched L FA-1-cytoskeletal complex assembled upon LFA-1 cross-linking and may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation. Expand
Inside the crawling T cell: leukocyte function-associated antigen-1 cross-linking is associated with microtubule-directed translocation of protein kinase C isoenzymes beta(I) and delta.
TLDR
It is found that in crawling T cells triggered via cross-linking of integrin LFA-1 two PKC isoenzymes, beta(I) and delta, are targeted to the cytoskeleton with specific localization corresponding to the microtubule-organizing center and microtubules, as detected by immunocytochemistry and immunoblotting. Expand
Microfluidic Enabling Platform for Cell-based Assays
We have developed proprietary technologies for executing continuous flow assays in biochips which mimic human capillaries. Such technologies are integral to the rapidly growing laboratoryExpand
Cell migration on topographical substrate . Measurement of the migration factors associated with two motile lymphoid cell types
  • Ann . Biomed . Eng .
  • 2003
Cell migration on topographical substrate. Measurement of the migration factors associated with two motile lymphoid cell
  • PRENDERGAST
  • 2003