A Multi-Source Portable Light Emitting Diode Spectrofluorometer

  title={A Multi-Source Portable Light Emitting Diode Spectrofluorometer},
  author={Safwan M. Obeidat and Baolong Bai and Gary D. Rayson and Dean M. Erson and Adam D Puscheck and Serge Yan Landau and Tzach Aharon Glasser},
  journal={Applied Spectroscopy},
  pages={327 - 332}
A portable luminescence spectrofluorometer weighing only 1.5 kg that uses multiple light emitting diodes (LEDs) as excitation sources was developed and evaluated. Excitation using a sequence of seven individual broad-band LED emission sources enabled the generation of excitation-emission spectra using a light weight (<1.5 kg) spectrometer. Limits of detection for rhodamine 6G, rhodamine B, and fluorescein were 2.9, 3.2, and 11.0 nM, respectively. Generation of excitation-emission matrices (EEMs… 
12 Citations

Figures and Tables from this paper

Hadamard-Transform Fluorescence Excitation-Emission-Matrix Spectroscopy.
The kinetics of the fluorescence of rhodamine B as a function of temperature and the acid-driven demetalation of chlorophyll-a into pheophytin-a was examined.
Portable system for measuring fluorescence signals of biological tissue
Preliminary results of ex vivo and in vivo fluorescence measurements of skin and brain tissues are presented and show that fluorescence and reflection could be used to diagnose and demarcat cancer boundaries.
Differentiating Among Plant Spectra by Combining pH Dependent Photoluminescence Spectroscopy with Multi-Way Principal Component Analysis (MPCA)
Photoluminescence spectroscopic probes offer the potential for differentiation among plant species in real-time. Spectral emission signatures (excitation at 365 nm) from three different pH (2.2, 7.5
A multi‐platform optical sensor for in situ sensing of water chemistry
A compact field‐deployable optical instrument using fluorescence, absorbance, and scattering to identify and quantify contaminants and natural substances in water bodies is described. The instrument
LED broadband spectral ellipsometer with switching of orthogonal polarization states
This paper describes an LED ellipsometer with a continuous wavelength range of 260–1000 nm and good technical characteristics. The measurement reproducibility and stability of the ellipsometric
Investigations on the Physical Structure and the Mechanism of Drug Release from an Enteric Matrix Microspheres with a Near-Zero-Order Release Kinetics Using SEM and Quantitative FTIR
Dissolution results indicated the ability of LS microspheres to minimize drug release during the acid stage, however, in the slightly basic medium that followed the acidic stage, the drug release was sustained and controlled in its kinetics and data fitted to Peppas equation indicated a case II transport suggesting that the drugrelease is mainly through swelling/erosion mechanism.


Light emitting diode excitation emission matrix fluorescence spectroscopy.
An excitation emission matrix (EEM) fluorescence instrument has been developed using a linear array of light emitting diodes (LED), which can be considered a hybrid between a spectroscopic and sensor system, as the broad LED excitation range produces a partially selective optical measurement.
MHz LED source for nanosecond fluorescence sensing
An inexpensive and portable pulsed light emitting diode (LED) source with variable repetition rate up to 10 MHz suitable for time-resolved fluorescence sensing in medical, environmental and
Portable LED-array VIS–NIR spectrophotometer/nephelometer
A portable instrument with a high-speed multiple-wavelength LED array source, and simultaneous detectors for absorbance and 90° nephelometry, was tested from 420 to 950 nm. Absorbance values and
Double‐LED Source for a Portable Photometer to Determine Iodine and Formaldehyde In Situ
Abstract An example that uses an LED (light emitting diode) as light source to miniaturize the conventional large spectrophotometer was demonstrated by the development of a portable photometer with
Light-Emitting Diodes as Sensors for Colorimetric Analyses
A new nondispersive colorimetric analyzer has been developed with the use of light-emitting diodes (LEDs) as optical sensors coupled to an artificial neural network (ANN). In this application,
Development of a battery‐operated portable synchronous luminescence spectrofluorometer
A battery‐operated portable synchronous luminescence spectrofluorometer was developed for on‐site analysis of groundwater or hazardous waste sites. The instrument is capable of either emission,
Photoluminescent Distinction among Plant Life Forms Using Phosphate Buffered Saline Extract Solutions
Photoluminescence of plant extract solutions has been investigated for discrimination of plant life forms (grasses, forbs, and shrubs) using principal component analysis (PCA). Clippings from each of
Remote detection of organochlorides with a fiber optic based sensor. III: Calibration and field evaluation of an improved chloroform fiber optic chemical sensor
Abstract A dedicated portable fluorometer has been used with a capillary-well fiber-optic chemical sensor (FOCS) to develop a calibration method for this sensor for gaseous chloroform. The FOCS has