A Mouse for All Reasons

  title={A Mouse for All Reasons},
  author={Francis S. Collins and Janet Rossant and Wolfgang Wurst},

Figures and Tables from this paper

Trends in large-scale mouse mutagenesis: from genetics to functional genomics

  • Y. Gondo
  • Biology
    Nature Reviews Genetics
  • 2008
The origins and rationale of large-scale mouse mutagenesis programmes are traced to develop a fundamental research infrastructure for mammalian functional genomics and to produce human disease models.

Dual RMCE for efficient re-engineering of mouse mutant alleles

dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

A conditional knockout resource for the genome-wide study of mouse gene function

High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome.

The mammalian gene function resource: the international knockout mouse consortium

The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research.

Identifying essential genes in mouse development via an ENU-based forward genetic approach.

An efficient and non-biased, N-ethyl-N-nitrosourea (ENU)-based forward genetic approach in mouse provides a unique tool for the identification of genes essential for development and adult physiology via ENU-based mutagenesis, which has become a powerful tool in deciphering gene functions.

A review of current large‐scale mouse knockout efforts

The knockout mouse project (KOMP) and the international gene trap consortium (IGTC) were initiated to create convenient resources for scientific research worldwide and knock out all the mouse genes.

Progress towards completing the mutant mouse null resource

It is found that there are less than 3400 mouse–human orthologs remaining in the genome without a targeted null allele that can be further prioritized to achieve the overall goal of the complete functional annotation of the protein-coding portion of a mammalian genome.



The Knockout Mouse Project

It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.

A public gene trap resource for mouse functional genomics

It is concluded that gene trapping is an effective strategy to mutate a substantial fraction of the genes in mice that compares favorably with gene-targeting approaches.

Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.

This work has used these directional recombination vectors to assemble the largest library of ES cell lines with conditional mutations in single genes yet assembled, presently totaling 1,000 unique genes.

A reliable lacZ expression reporter cassette for multipurpose, knockout‐first alleles

This work reports success with a “knockout‐first” strategy that ablates gene function by insertion of RNA processing signals without deletion of any of the target gene.

High-throughput engineering of the mouse genome coupled with high-resolution expression analysis

The development of a high-throughput and largely automated process that uses targeting vectors based on bacterial artificial chromosomes (BACs) that permits genetic alteration with nucleotide precision, is not limited by the size of desired deletions, does not depend on isogenicity or on positive–negative selection, and can precisely replace the gene of interest with a reporter that allows for high-resolution localization of target-gene expression.

Recombineering: a powerful new tool for mouse functional genomics

Recombineering facilitates many kinds of genomic experiment that have otherwise been difficult to carry out, and should enhance functional genomic studies by providing better mouse models and a more refined genetic analysis of the mouse genome.

Erratum: Corrigendum: The European dimension for the mouse genome mutagenesis program

The authors Johan Auwerx and Pierre Chambon are both affiliated with the Mouse Clinical Institute (MCI), Illkirch, Strasbourg, France.

Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes.

It is reported that homologous recombination using a promoterless gene trap vector ("targeting trapping") yields targeting frequencies averaging above 50%, a significant increase compared with current approaches.

A new logic for DNA engineering using recombination in Escherichia coli

A straightforward way to engineer DNA in E. coli using homologous recombination is described. The homologous recombination reaction uses RecE and RecT and is transferable between E. coli strains.


As part of the course at York each person is given the task of creating a character to present to the rest of the group. About ten weeks are allowed for its growth but hardly any of the preparation