A Modified RNA-Seq Approach for Whole Genome Sequencing of RNA Viruses from Faecal and Blood Samples

@inproceedings{Batty2013AMR,
  title={A Modified RNA-Seq Approach for Whole Genome Sequencing of RNA Viruses from Faecal and Blood Samples},
  author={Elizabeth M Batty and T. H. Nicholas Wong and Amy Trebes and Kar{\`e}ne Argoud and Moustafa Attar and David H. Buck and Camilla L. C. Ip and Tanya Golubchik and Madeleine L. Cule and Rory Bowden and Charis Manganis and Paul Klenerman and Eleanor Barnes and A. Sarah Walker and David H. Wyllie and Daniel J. Wilson and Kate E. Dingle and Tim E. A. Peto and Derrick W. Crook and Paolo A. Piazza},
  booktitle={PloS one},
  year={2013}
}
To date, very large scale sequencing of many clinically important RNA viruses has been complicated by their high population molecular variation, which creates challenges for polymerase chain reaction and sequencing primer design. Many RNA viruses are also difficult or currently not possible to culture, severely limiting the amount and purity of available starting material. Here, we describe a simple, novel, high-throughput approach to Norovirus and Hepatitis C virus whole genome sequence… CONTINUE READING
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