A Minimal Set of RNA Polymerase II Transcription Protein Interactions*

@article{Bushnell1996AMS,
  title={A Minimal Set of RNA Polymerase II Transcription Protein Interactions*},
  author={D. Bushnell and C. Bamdad and R. Kornberg},
  journal={The Journal of Biological Chemistry},
  year={1996},
  volume={271},
  pages={20170 - 20174}
}
All pairwise interactions of RNA polymerase II and general transcription factors (TF) IIB, E, F, and H have been quantitated by surface plasmon resonance with the use of a Ni2+ chelate on the sensor surface where necessary to attain higher sensitivity. Only 4 of 10 possible interactions were found above the detection limit: TFIIB, -E, and -F binding to RNA polymerase II and TFIIE binding to TFIIH. These four interactions constitute a minimal set for the formation of a transcription initiation… Expand
Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms
The structure of the general transcription factor IIB (TFIIB) in a complex with RNA polymerase II reveals three features crucial for transcription initiation: an N-terminal zinc ribbon domain ofExpand
Architecture of an RNA Polymerase II Transcription Pre-Initiation Complex
TLDR
The structure of a complete set of GTFs, assembled with pol II and promoter DNA in a 32-protein, 1.5 megaDalton “pre-initiation complex” (PIC), as revealed with cryo-electron microscopy (cryo-EM) and chemical cross-linking is reported on. Expand
The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex
TLDR
This work incorporated the non-natural photoreactive amino acid p-benzoyl-L-phenylalanine into the RNA polymerase II surface surrounding the central cleft formed by the Rpb1 and Rpb2 subunits to illuminate the structural organization of the PIC and the network of protein-protein interactions involved in transcription start site selection. Expand
Studies of the ncRNA binding site on RNA polymerase II and the sequence determinants of B2 RNA function as a repressor of transcription
TLDR
The studies detailed in this thesis examine the ncRNA binding site on Pol II, and the sequence determinants of B2 RNA that mediate repression of transcription in vitro. Expand
Formation and Crystallization of Yeast RNA Polymerase II Elongation Complexes*
TLDR
Minimal templates were devised for the efficient generation of yeast RNA polymerase II transcription elongation complexes that remained active in the crystal and, in the presence of nucleoside triphosphates, could efficiently extend the transcript in situ. Expand
Functional interactions of the Transcription Factor B during transcription initiation in Pyrococcus furiosus
TLDR
The results presented here are the first biochemical data on dynamic rearrangements of TFB during transcription initiation and transition to early elongation and further deepens the understanding of archaeal transcription processes and complements structural information derived from related eukaryotic organisms. Expand
Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery
  • M. Hampsey
  • Biology, Medicine
  • Microbiology and Molecular Biology Reviews
  • 1998
TLDR
This review focuses on the global effectors of RNA polymerase II transcription in yeast, including the general transcription factors, the coactivators, and the general repressors. Expand
The Rpb9 Subunit of RNA Polymerase II Binds Transcription Factor TFIIE and Interferes with the SAGA and Elongator Histone Acetyltransferases*
TLDR
Rpb9 has homology to the TFIIS elongation factor, but mutants inactivated for both proteins are indistinguishable from rpb9 single mutants, suggesting that Rpb9 contributes to the recruitment of TFIIE on RNA polymerase II. Expand
An Interaction between the N-terminal Region and the Core Domain of Yeast TFIIB Promotes the Formation of TATA-binding Protein-TFIIB-DNA Complexes*
TLDR
Results suggest that the intramolecular interaction in yeast TFIIB stabilizes a productive conformation of the protein for the association with promoter-bound TATA-binding protein. Expand
The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells.
TLDR
A model in which the TFIIB-RNAPII interaction controls the start site selection process by influencing the conformation of PIC prior to or during PIC assembly, as opposed to PIC stability is discussed. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 30 REFERENCES
Five intermediate complexes in transcription initiation by RNA polymerase II
TLDR
A native gel electrophoresis DNA binding assay was used to resolve complexes formed on the adenovirus Major Late Promoter by general transcription factors and RNA polymerase II, generating new complexes that contained accurately initiated transcripts associated with the transcription machinery and the template DNA. Expand
Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.
TLDR
Interestingly TFIIF was absolutely required for the formation of a preinitiation complex; however, it also affected the elongation phase of the transcription cycle, and was required for efficient elongation. Expand
Transcription factor IIE binds preferentially to RNA polymerase IIa and recruits TFIIH: a model for promoter clearance.
TLDR
TFIIE binds selectively to the nonphosphorylated form of RNA polymerase II (IIa) and that this interaction is mediated by the 56-kD subunit of TFIIE, which supports a model in which TF IIE plays a role in promoter clearance as well as in the recruitment of proteins required for transcription-coupled DNA repair. Expand
Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE.
Two general transcription factors (IIE and IIB) (TF) were purified from HeLa cell nuclear extracts and shown to be absolutely required, along with two additional factors (IIA and IID) and RNAExpand
RNA polymerase II initiation factor interactions and transcription start site selection.
TLDR
Exchange with components of a Saccharomyces cerevisiae system was undertaken to reveal the factor or factors responsible for the difference in location of the transcription start site, which shows that TFIIB and RNA polymerase II are solely responsible for determining the start site of transcription. Expand
Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.
TLDR
Two new factors required for transcription of class II genes have been identified and it was found that TFIIH and TFIIJ association with the preinitiation complex was ordered and required the previous assembly of a preinitation complex intermediate containing factors IID, IIB, IIF, IIE, and RNA polymerase II. Expand
Regulation of TFIIH ATPase and kinase activities by TFIIE during active initiation complex formation
TLDR
It is shown that human TFIIH has DNA-dependent ATPase activity and the stimulatory effect of TFIIE on both the ATPase and kinase activities is characterized, and it is demonstrated that extensive phosphorylation of RNA polymerase II occurs in aTFIIE-dependent manner in both the absence and presence of DNA. Expand
Common themes in assembly and function of eukaryotic transcription complexes.
TLDR
The complexity of the RNA polymerase II initiation pathway provides a multitude of potential targets for transcriptional activators and tight control over transcription initiation levels is afforded by multiple cofactors that both enhance and repress. Expand
TFIIF-TAF-RNA polymerase II connection.
TLDR
The cloning and sequencing of genes for a yeast protein that is the homolog of mammalian TFIIF are reported, which contains two subunits, Tfg1 and Tfg2, both of which are required for transcription, essential for yeast cell viability, and whose sequences exhibit significant similarity to those of the mammalian factor. Expand
The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex.
We found that transcription factor IIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). The resultingExpand
...
1
2
3
...