A Method to Rapidly and Accurately Compare the Relative Efficacies of Non-invasive Imaging Reporter Genes in a Mouse Model and its Application to Luciferase Reporters

@article{Gil2011AMT,
  title={A Method to Rapidly and Accurately Compare the Relative Efficacies of Non-invasive Imaging Reporter Genes in a Mouse Model and its Application to Luciferase Reporters},
  author={Jose S. Gil and Hidevaldo B. Machado and Harvey R. Herschman},
  journal={Molecular Imaging and Biology},
  year={2011},
  volume={14},
  pages={462-471}
}
Our goal is to develop a simple, quantitative, robust method to compare the efficacy of imaging reporter genes in culture and in vivo. We describe an adenoviral vector–liver transduction procedure and compare the luciferase reporter efficacies. Alternative reporter genes are expressed in a common adenoviral vector. Vector amounts used in vivo are based on cell culture titrations, ensuring that the same transduction efficacy is used for each vector. After imaging, in vivo and in vitro values are… CONTINUE READING
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