A Method to Quantify and Analyze the Foraging Activity of Honey Bees: Relevance to the Sublethal Effects Induced by Systemic Insecticides

A Method to Quantify and Analyze the Foraging Activity of Honey Bees: Relevance to the Sublethal Effects Induced by Systemic Insecticides},
  author={Marc Edouard Colin and Jean-Marc Bonmatin and I. Moineau and Cheryl Gaimon and Svante Brun and J. P. Vermandere},
  journal={Archives of Environmental Contamination and Toxicology},
The assessment of agropharmaceuticals’ side effects requires more realistic simulations of field conditions than those deduced from the dose-lethality relation obtained under laboratory conditions. Because the presence of sublethal doses or concentrations may also alter the behavior of foraging insects, we attempted to devise a quantifiable and accurate protocol for evidencing various alterations in free-flying bees. Such a protocol was illustrated by testing new classes of systemic… 

Honey Bees' Behavior Is Impaired by Chronic Exposure to the Neonicotinoid Thiacloprid in the Field.

Thiacloprid, a widely used cyano-substituted neonicotinoid thought to be less toxic to honey bees and of which use has increased in the last years, is presented for the first time.

Sublethal Effects of the Insecticide Pyrifluquinazon on the European Honey Bee (Hymenoptera: Apidae).

Results indicate that, if ingested at levels of 84 mg ai/liter, P QZ could have a negative effect on honey bee behavior; however, honey bee workers appear to be able to detect the presence of PQZ in their food and reject it.

Lethal and sublethal effects of thiamethoxam, a neonicotinoid molecule, on colony performance of A. mellifera

Under field conditions, average bee activity remained statistically low up to the 12th day after spray on mustard bloom in comparison to pre-count and control, and the effect of thiamethoxam under field conditions was less pronounced for bee mortality and colony parameters.

Neonicotinoids in bees: a review on concentrations, side-effects and risk assessment

The proposed risk assessment scheme for systemic compounds was shown to be applicable to assess the risk for side-effects of neonicotinoids as it considers the effect on different life stages and different levels of biological organization (organism versus colony).

A meta-analysis of experiments testing the effects of a neonicotinoid insecticide (imidacloprid) on honey bees

Findings raise renewed concern about the impact on honey bees of dietary imidacloprid, but because questions remain over the environmental relevance of predominantly laboratory-based results, targets for research are identified and procedural recommendations for future studies are provided.

Toxicity of neonicotinoid insecticides to honey bees: laboratory tests.

Commercial formulations, dispersed in sugar syrup and water, at the highest dose level marked on the label were used to carry out oral and indirect contact trials on each pesticide, causing higher mortality than the untreated controls only in oral toxicity tests when honey bees, which had starved for two hours, were used.

Toxicity of diafenthiuron to honey bees in laboratory, semi-field and field conditions.

Diafenthiuron was found to affect the foraging and homing behaviour of bees at sublethal concentrations, indicating that sublethal effects are more relevant in risk assessment than lethal and acute effects.

Modes of honeybees exposure to systemic insecticides: estimated amounts of contaminated pollen and nectar consumed by different categories of bees

Estimates for modes of honeybees exposure to systemic insecticides by estimating their pollen and nectar consumption are given for larvae and for the categories of adults which consume the highest amounts of - pollen, the nurse bees, and - nectar.

Imidacloprid Alters Foraging and Decreases Bee Avoidance of Predators

The results suggest that the effects of neonicotinoids on honey bee decision-making and other advanced cognitive functions should be explored and research should extend beyond the classic model, the European honey bee, to other important bee species.

Lethal and sublethal effects, and incomplete clearance of ingested imidacloprid in honey bees (Apis mellifera)

After exposure to syrup contaminated with 125 µg L−1 imidacloprid, honey bees experienced high mortality rates, had body residues of imidcloprid in the range 2.7–5.7 ng g−1 and exhibited abnormal behaviours that were significantly different from the controls, which contrast with the findings reported in the previous study.



Alteration of the homing‐flight in the honey bee Apis mellifera L. Exposed to sublethal dose of deltamethrin

Routine chemical analysis of exposed bees does not detect residues of deltamethrin 3 h after bee sublethal exposure, although bees evidenced alteration in the flight, and pyrethroids are known to disturb learning and memory.

Behaviour of Imidacloprid in Fields. Toxicity for Honey Bees

Following evidence for the intoxication of bees, the systemic insecticide imidacloprid was suspected from the mid nineties of having harmful effects. Recently, some studies have demonstrated that

The Influence of Time of Day on the Foraging Behavior of the Honeybee, Apis mellifera

The evidence gained in this study suggests that the diel change in accuracy exhibited by foraging groups, which persists after the removal of many potential environmental time cues, is an endogenously driven behavior pattern.

Effects of Sublethal Exposure to Diazinon on Longevity and Temporal Division of Labor in the Honey Bee (Hymenoptera: Apidae)

Longevity and foraging measures hold promise as potential methods of evaluating sublethal pesticide stress on the honey bee worker.

The Effect of Sublethal Doses of Parathion on Honeybee Behaviour. I. Oral Administration and the Communication Dance

The oral sublethal dose of parathion in honeybees was determined as less than 0–03 μg per bee, and there are suggestions that parathions at this level caused a temporary interference at an integrating centre outside the brain.

Secondary transmission of fipronil toxicity between oriental cockroaches Blatta orientalis L. in arenas.

Mixed colonies of adult male and female Oriental cockroaches were conditioned to a 12:12 h photocycle in arenas and third-instar nymphs were introduced and mortality was monitored over the subsequent 14 days, showing that no loss in the insecticidal activity of the cadavers occurred under these storage conditions.

Effects of Permethrin on the Behavior of Individually Tagged Honey Bees, Apis mellifera L. (Hymenoptera: Apidae)

Permethrin-treated adult bees exhibited a significantly higher percentage of time in self-cleaning, trembling dance, abdomen tucking, rotating, and cleaning of abdomen while rubbing hind legs together, than untreated bees.

Effect of Population Size on Brood Production, Worker Survival and Honey Gain in Colonies of Honeybees

SummaryThe effect of population size on brood production, worker survival and gain or loss of honey was studied in colonies of honeybees (Apis mellifera) in Louisiana, USA. About 11 kg of bees were

Sublethal effects of neurotoxic insecticides on insect behavior.

  • K. Haynes
  • Biology
    Annual review of entomology
  • 1988
Insecticides are the cornerstones upon which pest management practices are based, and are likely to remain so as long as effective and inexpensivechemicals are available.