A Metagenomic Survey of Microbes in Honey Bee Colony Collapse Disorder

  title={A Metagenomic Survey of Microbes in Honey Bee Colony Collapse Disorder},
  author={Diana L Cox-Foster and Sean Conlan and Edward C. Holmes and Gustavo F. Palacios and Jay D. Evans and Nancy A. Moran and Phenix-Lan Quan and Thomas Briese and Mady Hornig and David M. Geiser and Vincent G. Martinson and Dennis vanEngelsdorp and Abby L. Kalkstein and Andrew Drysdale and Jeffrey Hui and Junhui Zhai and Liwang Cui and Stephen K. Hutchison and Jan Fredrik Simons and Michael Wayland Egholm and Jeffery S. Pettis and W. Ian Lipkin},
  pages={283 - 287}
In colony collapse disorder (CCD), honey bee colonies inexplicably lose their workers. [] Key Method We used an unbiased metagenomic approach to survey microflora in CCD hives, normal hives, and imported royal jelly. Candidate pathogens were screened for significance of association with CCD by the examination of samples collected from several sites over a period of 3 years. One organism, Israeli acute paralysis virus of bees, was strongly correlated with CCD.

Colony Collapse Disorder of Honey Bee: A Neoteric Ruction in Global Apiculture

The study was undertaken to make a detailed idea on CCD, its impact, probable causes, economic importance, controversy etc by assembling the inferences of a number of global researchers.

In situ replication of honey bee colony collapse disorder

Data from this in situ study provide convincing evidence that exposure to sub-lethal levels of imidacloprid in HFCS causes honey bees to exhibit symptoms consistent to CCD 23 weeks post imidClinicalCD, and should be validated in future studies.

Pathogen Webs in Collapsing Honey Bee Colonies

Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies, and novel strains of the recently described Lake Sinai viruses were identified and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD.

Lack of Evidence for an Association between Iridovirus and Colony Collapse Disorder

A proteomics study implicated a double-stranded DNA virus, invertebrate iridescent virus (Family Iridoviridae) along with a microsporidium (Nosema sp.) as the cause of CCD, and surveyed healthy and CCD colonies from the United States and Israel and reanalyzed metagenomics data previously generated from RNA pools of C CD colonies for the presence of Irdovirus-like sequences.

Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)

Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD.

Iridovirus and Microsporidian Linked to Honey Bee Colony Decline

These findings implicate co-infection by IIV and Nosema with honey bee colony decline, giving credence to older research pointing to IIV, interacting with Nosema and mites, as probable cause of bee losses in the USA, Europe, and Asia.

Colony Collapse Disorder: A Descriptive Study

This is the first comprehensive survey of CCD-affected bee populations that suggests CCD involves an interaction between pathogens and other stress factors, and presents evidence that this condition is contagious or the result of exposure to a common risk factor.

Does infection by Nosema ceranae cause “Colony Collapse Disorder” in honey bees (Apis mellifera)?

N. ceranae is an emergent and potentially virulent pathogen of the honey bee (Apis meiiiferai) that has spread across the world in the last 10 or so years and observations of naturally infected colonies suggest that it leads to colony collapse in Spain.



Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field.

The gut of insects may harbour one of the largest reservoirs of a yet unexplored microbial diversity, and phylogenetic analyses indicated common bacterial phylotypes for all three bee species, e.g. those related to Simonsiella, Serratia, and Lactobacillus.

Bacterial community structures in honeybee intestines and their response to two insecticidal proteins.

Neither Bt-maize pollen nor high concentrations of Cry1Ab significantly affected bacterial communities in honeybee intestines, and total bacterial community structures may not be a sensitive indicator for providing evidence for the impact of insecticidal proteins on honeybees at sublethal levels.

Conditional immune-gene suppression of honeybees parasitized by Varroa mites

Bees exposed to low or moderate number of mites had fewer immune-related transcripts than pupae that were never parasitized or pupae with high mite loads, and all bees tested negative for acute paralysis and Kashmir bee viruses known to be vectored by V. destructor.

Effects of coumaphos on queen rearing in the honey bee, Apis mellifera

Young honey bee larvae were transferred into the queen cups containing known concentrations of the organophosphate pesticide coumaphos and placed in queenless colonies and examined ten days later to determine the rate of rejection or acceptance as indicated by a mature sealed queen cell.

What's Killing American Honey Bees?

American beekeepers reported unusually high rates of colony loss in early 2007 as bees broke from their overwintering clusters. Researchers are struggling to explain what's behind this mysterious

Diversity of Insect Trypanosomatids Assessed from the Spliced Leader RNA and 5S rRNA Genes and Intergenic Regions1

The data revealed additional discrepancies between molecular phylogenetic data and cell morphology, rendering current trypanosomatid taxonomy unreliable, and several isolates seem to belong to a single species with an unexpectedly wide host and geographical range.

Phylogeny of Trypanosomatidae and Bodonidae (Kinetoplastida) based on 18S rRNA: evidence for paraphyly of Trypanosoma and six other genera.

The results suggested that parasitism of vertebrates has probably arisen independently a number of times within the Trypanosomatidae.