A Metagenomic Survey of Microbes in Honey Bee Colony Collapse Disorder

@article{CoxFoster2007AMS,
  title={A Metagenomic Survey of Microbes in Honey Bee Colony Collapse Disorder},
  author={Diana L Cox-Foster and Sean Conlan and Edward C. Holmes and Gustavo F. Palacios and Jay D. Evans and Nancy A. Moran and Phenix-Lan Quan and Thomas Briese and Mady Hornig and David M. Geiser and Vincent G Martinson and Dennis vanEngelsdorp and Abby L. Kalkstein and Andrew Drysdale and Jeffrey Hui and Junhui Zhai and Liwang Cui and Stephen K. Hutchison and Jan Fredrik Simons and Michael Wayland Egholm and Jeffery S Pettis and W. Ian Lipkin},
  journal={Science},
  year={2007},
  volume={318},
  pages={283 - 287}
}
In colony collapse disorder (CCD), honey bee colonies inexplicably lose their workers. [...] Key Method We used an unbiased metagenomic approach to survey microflora in CCD hives, normal hives, and imported royal jelly. Candidate pathogens were screened for significance of association with CCD by the examination of samples collected from several sites over a period of 3 years. One organism, Israeli acute paralysis virus of bees, was strongly correlated with CCD.Expand
In situ replication of honey bee colony collapse disorder
TLDR
Data from this in situ study provide convincing evidence that exposure to sub-lethal levels of imidacloprid in HFCS causes honey bees to exhibit symptoms consistent to CCD 23 weeks post imidClinicalCD, and should be validated in future studies. Expand
Pathogen Webs in Collapsing Honey Bee Colonies
TLDR
Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies, and novel strains of the recently described Lake Sinai viruses were identified and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. Expand
Lack of Evidence for an Association between Iridovirus and Colony Collapse Disorder
TLDR
A proteomics study implicated a double-stranded DNA virus, invertebrate iridescent virus (Family Iridoviridae) along with a microsporidium (Nosema sp.) as the cause of CCD, and surveyed healthy and CCD colonies from the United States and Israel and reanalyzed metagenomics data previously generated from RNA pools of C CD colonies for the presence of Irdovirus-like sequences. Expand
Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)
TLDR
Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD. Expand
Iridovirus and Microsporidian Linked to Honey Bee Colony Decline
TLDR
These findings implicate co-infection by IIV and Nosema with honey bee colony decline, giving credence to older research pointing to IIV, interacting with Nosema and mites, as probable cause of bee losses in the USA, Europe, and Asia. Expand
Characterization of viral siRNA populations in honey bee colony collapse disorder.
TLDR
It is suggested that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses, similar to that observed in colonies experimentally infected with IAPV. Expand
Colony Collapse Disorder: A Descriptive Study
TLDR
This is the first comprehensive survey of CCD-affected bee populations that suggests CCD involves an interaction between pathogens and other stress factors, and presents evidence that this condition is contagious or the result of exposure to a common risk factor. Expand
Does infection by Nosema ceranae cause “Colony Collapse Disorder” in honey bees (Apis mellifera)?
TLDR
N. ceranae is an emergent and potentially virulent pathogen of the honey bee (Apis meiiiferai) that has spread across the world in the last 10 or so years and observations of naturally infected colonies suggest that it leads to colony collapse in Spain. Expand
Honey bee colony losses and associated viruses.
TLDR
The existing correlative evidence provided by monitoring studies is reviewed which identified members of the deformed wing virus and acute bee paralysis virus clades as lethal pathogens for entire colonies, and identified novel viruses whose impact on honey bee health remains elusive. Expand
Dynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies with Colony Collapse Disorder
TLDR
It is found that IAPV was the dominant pathogen and it replicated actively in the colonies: viral titers decreased from April to September and increased from September to December, indicating that once acquired and induced to replication it acts as an infectious factor that affects the health of the colonies and may determine their survival. Expand
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