A Medipix quantum area detector allows rotation electron diffraction data collection from submicrometre three-dimensional protein crystals

@inproceedings{Nederlof2013AMQ,
  title={A Medipix quantum area detector allows rotation electron diffraction data collection from submicrometre three-dimensional protein crystals},
  author={Igor Nederlof and Eric van Genderen and Yao-Wang Li and Jan Pieter Abrahams},
  booktitle={Acta crystallographica. Section D, Biological crystallography},
  year={2013}
}
When protein crystals are submicrometre-sized, X-ray radiation damage precludes conventional diffraction data collection. For crystals that are of the order of 100 nm in size, at best only single-shot diffraction patterns can be collected and rotation data collection has not been possible, irrespective of the diffraction technique used. Here, it is shown that at a very low electron dose (at most 0.1 e(-) Å(-2)), a Medipix2 quantum area detector is sufficiently sensitive to allow the collection… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-4 of 4 references

Evolving Methods for Macromolecular Crystallography

A.G.W. Leslie, H. R. Powell
2007
View 9 Excerpts
Highly Influenced

Q

R. Henderson
Rev. Biophys. 28, 171–193. • 1995
View 1 Excerpt

Jnt CCP4/ESF–EACBM Newsl

J. P. Abrahams
Protein • 1993
View 2 Excerpts

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