A Mathematical Model for Suppression Subtractive Hybridization

  title={A Mathematical Model for Suppression Subtractive Hybridization},
  author={Chetan J. Gadgil and Anette Rink and Craig W. Beattie and Wei-Shou Hu},
  journal={Comparative and Functional Genomics},
  pages={405 - 422}
Suppression subtractive hybridization (SSH) is frequently used to unearth differentially expressed genes on a whole-genome scale. Its versatility is based on combining cDNA library subtraction and normalization, which allows the isolation of sequences of varying degrees of abundance and differential expression. SSH is a complex process with many adjustable parameters that affect the outcome of gene isolation.We present a mathematical model of SSH based on DNA hybridization kinetics for… CONTINUE READING

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