A Lymphocyte-stimulating Factor produced in vitro

@article{Gordon1965ALF,
  title={A Lymphocyte-stimulating Factor produced in vitro},
  author={John R. Gordon and Lloyd D. Maclean},
  journal={Nature},
  year={1965},
  volume={208},
  pages={795-796}
}
Bain, Vas and Lowenstein1 have recently reported that when peripheral leucocytes of two individuals are cultured together, some of the cells enlarge and undergo mitosis. Bach and Hirschhorn2 have further shown that not only intact cells, but also extracts of leucocytes, disrupted by freezing and thawing, could stimulate cells to divide. Experiments reported in this communication demonstrate that cell-free media obtained from leucocytes in culture contain mitogenic factor(s), and suggest that… 

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References

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THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES.

TLDR
It is suggested that the reaction in leukocyte mixtures may be related to homograft immunity and studies with mixtures of leukocytes from pairs of identical and nonidentical twins indicate that the response may berelated to genetic differences between the two subjects.

Lymphocyte Interaction: A Potential Histocompatibility Test in vitro

TLDR
Lymphocytes from two unrelated individuals, cultured together in the same tube, undergo morphological transformation to large cells and divide and the degree of cross-reactivity of grafts from the two individuals placed on a third unrelated recipient is correlated.