A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells

  title={A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells},
  author={Christian Schulz and Elisa Gomez Perdiguero and Laurent Chorro and Heather L. Szabo-Rogers and Nicolas Cagnard and Katrin Kierdorf and Marco Prinz and Bishan Wu and Sten Eirik W. Jacobsen and Jeffrey W. Pollard and Jon Frampton and Karen J. Liu and Fr{\'e}d{\'e}ric Geissmann},
  pages={86 - 90}
Macrophage Development Rewritten Macrophages provide protection against a wide variety of infections and critically shape the inflammatory environment in many tissues. These cells come in many flavors, as determined by differences in gene expression, cell surface phenotype and specific function. Schulz et al. (p. 86, published online 22 March) investigated whether adult macrophages all share a common developmental origin. Immune cells, including most macrophages, are widely thought to arise… 

Myb-independent macrophages: a family of cells that develops with their tissue of residence and is involved in its homeostasis.

A genetic and molecular dissection of resident macrophage functions will reveal their roles in tissue metabolism and the maintenance of homeostasis independently of the extravasation of inflammatory leukocytes, and in the control of the recruitment of BM-derived cells in overt inflammation.

Development and maintainance of resident macrophages

The purpose of this Review is to present and discuss current knowledge on the developmental biology of macrophages, as it underlies the concept of a layered myeloid system composed of ‘resident’ macrophage that mostly originate from yolk sac progenitors and of ’passenger’ or ‘transitory’ myeloids cells that originate and renew from bone marrow hematopoietic stem cells.

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry.

A tamoxifen-inducible fate mapping protocol is established based on expression of the macrophage cytokine receptor Csf1r promoter to characterize EMP and EMP-derived cells in vivo by flow cytometry.

Differences in Cell-Intrinsic Inflammatory Programs of Yolk Sac and Bone Marrow Macrophages

While YS and BM macrophages demonstrate close similarities in terms of cellular growth, differentiation, cell death susceptibility and phagocytic properties, they display differences in cell metabolism, expression of inflammatory markers and inflammasome activation.

Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors

It is shown in mice that the vast majority of adult tissue-resident macrophages originate from a Tie2+ (also known as Tek) cellular pathway generating Csf1r+ erythro-myeloid progenitors (EMPs) distinct from HSCs.

CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages

It is shown that combining FLT3 and CSF1R-deficiencies results in specific and complete abrogation of spleen DCs in vivo, providing a novel regulatory principle that may also be important for the differentiation of other hematopoietic cell types.

Ongoing Production of Tissue-Resident Macrophages from Hematopoietic Stem Cells in Healthy Adult Macaques

It is found that, in all anatomic sites the authors studied, HSPC contribute to tissue-resident macrophage populations, and their clonotypic profile is dynamic and overlaps significantly with the clonal hierarchy of contemporaneous monocytes in peripheral blood.

Tissue-Resident Macrophage Development and Function

The developmental origins of tissue-resident macrophages, their molecular regulation in specific tissues, and their impact on embryonic development and postnatal homeostasis are discussed.

Ontogeny of Tissue-Resident Macrophages

Evidence now shows that certain macrophage populations are in fact independent from monocyte and even from adult bone marrow hematopoiesis, and derive from sequential seeding of tissues by two precursors during embryonic development.

Early hematopoiesis and macrophage development.




A Clonogenic Bone Marrow Progenitor Specific for Macrophages and Dendritic Cells

The isolation and clonal analysis of a mouse bone marrow progenitor that is specific for monocytes, several macrophage subsets, and resident spleen DCs in vivo is described, providing a cellular and molecular basis for the concept of the mononuclear phagocyte system.

MafB/c-Maf Deficiency Enables Self-Renewal of Differentiated Functional Macrophages

It is reported that combined deficiency for the transcription factors MafB and c-Maf enables extended expansion of mature monocytes and macrophages in culture without loss of differentiated phenotype and function and thus appears possible to amplify functional differentiated cells without malignant transformation or stem cell intermediates.

Langerhans cell (LC) proliferation mediates neonatal development, homeostasis, and inflammation-associated expansion of the epidermal LC network

Most tissues develop from stem cells and precursors that undergo differentiation as their proliferative potential decreases. Mature differentiated cells rarely proliferate and are replaced at the end

Kupffer cell heterogeneity: functional properties of bone marrow derived and sessile hepatic macrophages.

This study uses both flow cytometry and immunohistochemistry to distinguish 2 subsets of Kupffer cells that were revealed in the context both of bone marrow transplantation and of orthotopic liver transplantation, and proposes the name "sessile" for the radioresistant Kupfer cells that do not participate in immunoinflammatory reactions.

Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages

Results identify microglia as an ontogenically distinct population in the mononuclear phagocyte system and have implications for the use of embryonically derived microglial progenitors for the treatment of various brain disorders.

PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesis

Findings emphasize the distinct nature of adult hematopoiesis and reveal that PU.1 regulates the specification of the multipotent lymphoid and myeloid compartments and restrains, rather than promotes, granulopoiedis.

Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1.

The results support previous evidence that yolk sac-derived fetal phagocytes are functionally distinct from those arising in the liver and develop via a different pathway and support the hypothesis that a similar transition in phenotype occurs in myelopoiesis.

Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities.

While the PU.1 protein appears not to be essential for myeloid and lymphoid lineage commitment, it is absolutely required for the normal differentiation of B cells and macrophages.

Flk-2 is a marker in hematopoietic stem cell differentiation: A simple method to isolate long-term stem cells

To establish whether the Flk-2/Flt3 receptor tyrosine kinase was expressed on the most primitive LT-HSCs, highly purified multipotent stem and progenitor cells were sorted and used in competitive reconstitution assays.

Hematopoietic stem cells derive directly from aortic endothelium during development

The unique strengths of the zebrafish embryo are used to image directly the generation of HSCs from the ventral wall of the dorsal aorta and it is demonstrated that the H SCs generated from haemogenic endothelium are the lineal founders of the adult haematopoietic system.