A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter.

@article{Perelle2004ALR,
  title={A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter.},
  author={Sylvie Perelle and Mathilde Hasseldam Josefsen and Jeffrey Hoorfar and Françoise Dilasser and J. A. Grout and Patrick Fach},
  journal={Molecular and cellular probes},
  year={2004},
  volume={18 5},
  pages={321-7}
}
In a previous study, we reported the performance of a PCR assay amplifying 287-bp of the 16S rRNA gene of thermo-tolerant Campylobacter (C. jejuni, C. lari, C. coli) through an international ring-trial involving 12 participating laboratories. Based on the validated set of primers, a LightCycler real-time PCR assay (LC-PCR), which used fluorescent hybridization probes was developed. The test incorporated an internal amplification control co-amplified with the 16S rRNA gene of Campylobacter to… CONTINUE READING

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