A Leu → His substitution at residue 93 in human corticosteroid binding globulin results in reduced affinity for cortisol

@article{Smith1992AL,
  title={A Leu → His substitution at residue 93 in human corticosteroid binding globulin results in reduced affinity for cortisol},
  author={Carolyn L. Smith and Stephen G. A. Power and Geoffrey L. Hammond},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={1992},
  volume={42},
  pages={671-676}
}
A steroid binding capacity assay and a radioimmunoassay were both used to measure corticosteroid binding globulin (CBG) in serum samples from 22 patients with sepsis. An approximately 50% discordancy between the two values in one patient suggested the presence of a CBG variant with reduced affinity for cortisol, and this was confirmed by Scatchard analysis. We therefore used the polymerase chain reaction to amplify exons that encode for human CBG from the genomic DNA of this patient. This… Expand
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