A LED light calibration source for dual-wavelength microscopy.


An LED light source for dual-wavelength calibration of light microscope recording systems is described. A 1 mm2 circular field of bi-chromatic illumination is formed by passing the crossed beams of two light emitting diodes (LEDS) through an optical diffuser and circular mask. The source is constructed using LEDs which emit light at wavelengths overlapping… (More)


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