A Kunitz-type glycosylated elastase inhibitor with one disulfide bridge.

@article{Sumikawa2006AKG,
  title={A Kunitz-type glycosylated elastase inhibitor with one disulfide bridge.},
  author={Joana Tomomi Sumikawa and Adriana Miti Nakahata and Hans Fritz and Reinhardt Mentele and Misako Uemura Sampaio and Maria Luiza Vilela Oliva},
  journal={Planta medica},
  year={2006},
  volume={72 5},
  pages={393-7}
}
A glycosylated Bauhinia rufa elastase inhibitor (gBrEI) was purified and characterized using acetone precipitation, affinity chromatography on concanavalin A-Sepharose, ion-exchange chromatography on a HiTrap Q column, size exclusion chromatography on a Superdex 200 column and reverse-phase chromatography on a C18 column. gBrEI inhibited pancreatic porcine elastase with an equilibrium dissociation constant (K(i)) of 6.18 x 10(-8) M, but it did not inhibit human neutrophil elastase, bovine… CONTINUE READING