A Kluyveromyces lactis mycocin active at neutral pH

  title={A Kluyveromyces lactis mycocin active at neutral pH},
  author={Wladyslav I. Golubev},
  pages={290 - 294}
A strain of Kluyveromyces lactis was found to secrete a fungicidal mycocin active in the pH range from 6 to 9 and exhibiting the highest activity at pH of approximately 7. A few yeast species of the families Saccharomycetaceae and Wickerhamomycetaceae were sensitive to the mycocin. Some genera and species were heterogeneous in this respect. UV treatment of the mycocinogenic strain resulted in loss of its antifungal activity. Although prokaryotes were not sensitive to the mycocin, the strain… 

Native Killer Yeasts as Biocontrol Agents of Postharvest Fungal Diseases in Lemons

The native killer yeasts studied in this work showed to be an effective alternative for the biocontrol of postharvest fungal infections of lemons and could be promising agents for the development of commercial products for the biological control industry.


The results of the present study suggest that killer toxins produced by probiotic yeast strains can be used as an antimicrobial agent to control the microbial contamination in the food industry.

Characterisation of the microbiota of traditional fermented beverages and screening these and other populations for novel antimicrobial producers and gene clusters

Characterisation of the microbiota of traditional fermented beverages and screening these and other populations for novel antimicrobial producers and gene clusters Author(s) Marsh, Alan J.


The purpose of the present review is to explore the updated information on the efficacy of various yeast strains as probiotics for human welfare.



Action spectrum of Kluyveromyces lactis mycocins

New mycocinogenic strains of the yeast Kluyveromyces lactis were found that have fungicidic activity at pH from 5 to 7 and ones sensitive to theseMycocins were revealed mainly in the families Saccharomycetaceae and Wickerhamomycettales.

Heterologous protein production in the yeast Kluyveromyces lactis.

The various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression are reviewed and data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression is presented.

Selection and Study of Potent Lactose-Fermenting Yeasts

Whey-fermenting Kluyveromyces cultures were revealed among 105 yeast strains assimilating lactose, and most strains were resistant to cycloheximide and grew in medium containing glucose, NaCl, and ethanol at concentrations of up to 50, 11–12, and 10–12%, respectively.

Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts

The results suggested that killer activity of yeasts might operate over bacteria and it could be used for the biocontrol of contaminating bacteria from alcoholic fermentation if additional tests on toxin application in fermentation shown to be successful.

Killer toxins of certain yeast strains have potential growth inhibitory activity on gram-positive pathogenic bacteria.

Yeast killer toxins were found to be active only on Gram-positive bacterial cell types, while the killer toxins of Hansenula anamola, Hansenula mrakii, Candida tropicalis, Kluyveromyces drosphilarum and Kluy Veromyces lactis showed potential growth inhibitory effects on Gram -positive pathogenic bacteria.

The plasmid‐encoded killer system of Kluyveromyces lactis: A review

La structure molecule et l'assemblage de the toxine sont egalement etudies au systeme killer de Kluyveromyces lactis.

Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis.

Lactis toxin was effective with sensitive strains of S. cerevisiae in a relatively wider pH range between 4 and 8; it was stable for several months at pH 6.0 when stored below -20 degrees C.

Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character

Two linear deoxyribonucleic acid plasmids were isolated from the yeast Kluyveromyces lactis IFO 1267 and a single chromosomal gene was found which was responsible for the resistance to the K. lactis killer.

Mating-type locus control of killer toxins from Kluyveromyces lactis and Pichia acaciae.

A novel effect of yeast killer toxins on mating and sexual reproduction that is independent of their impact on cellular proliferation and cell-cycle progression is revealed.

Emerging importance of multidrug-resistant Acinetobacter species and Stenotrophomonas maltophilia as pathogens in seriously ill patients: geographic patterns, epidemiological features, and trends in the SENTRY Antimicrobial Surveillance Program (1997-1999).

The geographic differences in resistance patterns among Acinetobacter species and S. maltophilia isolates observed in this study emphasize the importance of local surveillance in determining the most adequate therapy for acinetobacterial infections and the possible clonal, epidemic nature of occurrence.