A Key Role for Diacylglycerol Lipase-α in Metabotropic Glutamate Receptor-Dependent Endocannabinoid Mobilization

@article{Jung2007AKR,
  title={A Key Role for Diacylglycerol Lipase-$\alpha$ in Metabotropic Glutamate Receptor-Dependent Endocannabinoid Mobilization},
  author={Kwang-Mook Jung and Giuseppe Astarita and Chenggang Zhu and Matthew Wallace and Ken Mackie and Daniele Piomelli},
  journal={Molecular Pharmacology},
  year={2007},
  volume={72},
  pages={612 - 621}
}
Activation of group I metabotropic glutamate (mGlu) receptors recruits the endocannabinoid system to produce both short- and long-term changes in synaptic strength in many regions of the brain. Although there is evidence that the endocannabinoid 2-arachidonoylglycerol (2-AG) mediates this process, the molecular mechanism underlying 2-AG mobilization remains unclear. In the present study, we used a combination of genetic and targeted lipidomic approaches to investigate the role of the… 
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160 Citations

Activation of Type 5 Metabotropic Glutamate Receptors and Diacylglycerol Lipase-α Initiates 2-Arachidonoylglycerol Formation and Endocannabinoid-Mediated Analgesia

The results indicate that activation of the postsynaptic mGlu5–DGL-α cascade triggers retrograde 2-AG signaling in vivo, which is required for endocannabinoid-mediated stress-induced analgesia.

CaMKII is a novel regulator of diacylglycerol lipase-α and striatal endocannabinoid signaling

A functional interaction between the 2-AG synthetic enzyme diacylglycerol lipase-α (DGLα) and calcium/calmodulin dependent protein kinase II (CaMKII) is identified, providing mechanistic insights into the molecular regulation of striatal endocannabinoid signaling with implications for physiological control of motor function.

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Rapid advances in enzymological research are outlined on the biosynthetic and degradative pathways of 2-AG, a monoacylglycerol molecule containing an esterified arachidonic acid chain at sn-2 position of the glycerol backbone.

Molecular Reconstruction of mGluR5a-Mediated Endocannabinoid Signaling Cascade in Single Rat Sympathetic Neurons

A model system based on isolated rat sympathetic neurons is constructed, in which an eCB signaling cascade could be studied in a reduced, spatially compact, and genetically malleable system and provides a platform for testing candidate molecules underlying facilitation of eCB transport across the plasma membrane.

Diacylglycerol Lipase-α and -β Control Neurite Outgrowth in Neuro-2a Cells through Distinct Molecular Mechanisms

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Brain regional cannabinoid CB(1) receptor signalling and alternative enzymatic pathways for 2-arachidonoylglycerol generation in brain sections of diacylglycerol lipase deficient mice.

DAGLβ Inhibition Perturbs a Lipid Network Involved in Macrophage Inflammatory Responses

It is shown that DAGLβ inactivation lowers 2-AG, as well as arachidonic acid and eicosanoids, in mouse peritoneal macrophages in a manner that is distinct and complementary to disruption of cytosolic phospholipase-A2 (PLA2G4A).

cAMP Signaling–Mediated Phosphorylation of Diacylglycerol Lipase α Regulates Interaction With Ankyrin-G and Dendritic Spine Morphology

Diacylglycerol Lipase- (cid:1) and - (cid:2) Control Neurite Outgrowth in Neuro-2a Cells through Distinct Molecular Mechanisms □

The results indicate that RA-induced DGL expression is re- quired for neurite outgrowth of Neuro-2a cells and suggest that DGL- ( cid:1) and - (cid:2) may regulate neuriteOutgrowth by engaging temporally and spatially distinct molecular pathways.

Possible inhibitory roles of endogenous 2-arachidonoylglycerol during corticotropin-releasing factor-induced activation of central sympatho-adrenomedullary outflow in anesthetized rats.

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