A Kazal-type serine proteinase inhibitor from chicken liver (clTI-1): purification, primary structure, and inhibitory properties.

@article{Kubiak2009AKS,
  title={A Kazal-type serine proteinase inhibitor from chicken liver (clTI-1): purification, primary structure, and inhibitory properties.},
  author={Agnieszka Kubiak and Piotr Jakimowicz and Antoni Polanowski},
  journal={International journal of biological macromolecules},
  year={2009},
  volume={45 2},
  pages={194-9}
}
Low-molecular-mass trypsin inhibitor (clTI-1; chicken liver Trypsin Inhibitor-1) was purified from chicken liver by extraction with perchloric acid, ammonium sulfate precipitation, a combination of ethanol-acetone fractionation followed by gel filtration, ion-exchange chromatography and RP-HPLC on a C18 column. The inhibitor occurs in two isoforms with molecular masses of 5938.56 and 6026.29 Da (determined by MALDI TOFF mass spectrometry). The complete amino acid sequences of both isoforms were… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-7 of 7 references

Res

K. Matsuda, M. Ogawa, A. Murata, T. Kitahara, G. Kosaki
Commun. Chem. Pathol. Pharmacol 40 (1983) 301–305. [ [ [ ical Macromolecules 45 • 2009
View 1 Excerpt

In Vitro Cell Dev

A. E. Arias, T. Boldicke, M. Bendayan
Biol. 29A • 1993
View 1 Excerpt

Scand

A. Lasson, A. Borgstrom, K. Ohlsson
J. Gastroenterol. 21 • 1986
View 1 Excerpt

Am

K. Matsuda, M. Ogawa, +4 authors T. Mori
J. Gastroenterol. 80 • 1985

Hoppe Seylers Z Physiol

B. Meloun, D. Cechova, V. Jonakova
Chem. 364 • 1983
View 1 Excerpt

Clin

T. Kitahara, Y. Takatsuka, +3 authors G. Kosaki
Chim. Acta 103 • 1980
View 1 Excerpt

Physiol

A. Eddeland, K. Ohlsson, Z. Hoppe Seylers
Chem. 359 • 1978
View 1 Excerpt

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