A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seeds.

@article{Petruccelli2006AKM,
  title={A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seeds.},
  author={Silvana Petruccelli and Marisa S Otegui and Fabricio Jorge Lareu and Olivia Tran Dinh and A C Fitchette and Ariana Circosta and Mart{\'i}n Rumbo and Muriel Bardor and Rosa C{\'a}rcamo and V{\'e}ronique Gomord and Roger N. Beachy},
  journal={Plant biotechnology journal},
  year={2006},
  volume={4 5},
  pages={511-27}
}
Transgenic plants are attractive biological systems for the large-scale production of pharmaceutical proteins. In particular, seeds offer special advantages, such as ease of handling and long-term stable storage. Nevertheless, most of the studies of the expression of antibodies in plants have been performed in leaves. We report the expression of a secreted (sec-Ab) or KDEL-tagged (Ab-KDEL) mutant of the 14D9 monoclonal antibody in transgenic tobacco leaves and seeds. Although the KDEL sequence… CONTINUE READING

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A KDEL - tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves , but is both partially secreted and sorted to protein storage vacuoles in seeds .
A KDEL - tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves , but is both partially secreted and sorted to protein storage vacuoles in seeds .
Although the KDEL sequence has little effect on the accumulation of the antibody in leaves , it leads to a higher antibody yield in seeds .
Although the KDEL sequence has little effect on the accumulation of the antibody in leaves , it leads to a higher antibody yield in seeds .
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