A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome

@article{Stelzl2005AHP,
  title={A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome},
  author={Ulrich Stelzl and Uwe Worm and Maciej M. Lalowski and Christian Haenig and Felix H. Brembeck and Heike Goehler and Martin Stroedicke and Martina Zenkner and Anke Schoenherr and Susanne Koeppen and Jan Timm and Sascha Mintzlaff and Claudia Abraham and Nicole Bock and S. Kietzmann and Astrid Goedde and Engin Toks{\"o}z and Anja Droege and Sylvia Krobitsch and Bernhard Korn and Walter Birchmeier and Hans Lehrach and Erich E. Wanker},
  journal={Cell},
  year={2005},
  volume={122},
  pages={957-968}
}
Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the… Expand
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